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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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46권 10호

치수의 구조와 생리, 진단
김영재(서울대학교 치과대학 소아치과학교실) pp.600-607 https://doi.org/10.22974/jkda.2008.46.10.001
유치의 치수치료
최남기(전남대학교) pp.608-613 https://doi.org/10.22974/jkda.2008.46.10.002
미성숙영구치의 치수치료
이상훈(서울대학교 치과대학 소아치과학교실) pp.614-622 https://doi.org/10.22974/jkda.2008.46.10.003
Zoledronate가 UMR-106 세포의 증식과 조골세포 형성에 미치는 영향
정기훈(경희대학교 일반대학원 치의학과 구강악안면외과학) ; 류동목(경희대학교) ; 지유진(경희대학교 치의학전문대학원) ; 이덕원(경희대학교 일반대학원 치의학과 구강악안면외과학) ; 이현우(경희대학교 치의학전문대학원 치과약리학교실) pp.623-632 https://doi.org/10.22974/jkda.2008.46.10.004
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Abstract

Purpose: The purpose of this study is to identify the effect of zoledronate(Zometa®), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast. Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x 104 cells per plates. Each plates were incubated with 5% CO2 incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, 3μM of zoledronate(Zometa®), every 2 days, for 12 days. “Control group” was plates not added with zoledronate(0μM), and “experiment group” were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, 3μM). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405nm with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast. Results: Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased. Conclusion: From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.

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