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open access
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ISSN : 0376-4672
Dentin surface of non-carious lesion is usually attached with oral biofilm. The biofilm should be removed before application of restorative material, because it may reduce the bond strength of adhesive system. The aim of this study was to evaluate the microtensile bond strength, when the biofilm was removed with brush or bur. Twenty extracted human third molars were sectioned horizontally to obtain dentin surface. Specimen were divided randomly into four group. Biofilm formation was performed in three group, except for Group 1 (negative control). Biofilm was removed as follows: Group 3, using ICB brush; Group 4, using low-speed round bur #2. Group 2 (positive control) was not removed Biofilm. And in all four groups, the adhesive system (Optibond FL, Kerr) was applied to etched dentin surface, and resin composite was built up in three 1mm increments. After 24 hour storage in distilled water, the teeth were perpendicularly sectioned to obtain beams (1 x 1 mm2). Microtensile bond strength was measured and the data were statistically analyzed using one-way ANOVA and Tukey’s post hoc test (p<0.05). Group 4 showed the highest microtensile bond strength (p<0.05), Group 3 showed no significant improvements when compared to Group 1. Group 2 showed lowest microtensile bond strength (p<0.05). When restoring a non-carious cervical lesion, it is essential to remove the biofilm present on the dentin surface. In addition, in the method of removing the biofilm, both the brush removal method and the bur removal method were effective.
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