Effects of Insulin-like Action and Insulin Sensitizing on 3T3-L1 Adipocytes from Coicis Semen

김종욱; 최용휴; 주영승; 박선민; 이미영; 김호경; 김홍준; 고병섭

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P-ISSN 1010-0695
E-ISSN 2288-3339

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23권 1호

Effects of Insulin-like Action and Insulin Sensitizing on 3T3-L1 Adipocytes from Coicis Semen

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339

2002, v.23 no.1, pp.83-91

김종욱 (우석대학교)
최용휴 (우석대학교)
주영승 (우석대학교)
박선민 (호서대학교)
이미영 (한국한의학연구원)
김호경 (한국한의학연구원)
김홍준 (한국한의학연구원)
고병섭 (한국한의학연구원)

김종욱, 최용휴, 주영승, 박선민, 이미영, 김호경, 김홍준, & 고병섭. (2002). 薏苡仁이 3T3-L1 Adipocytes에서 인슐린성 작용과 인슐린 민감성에 미치는 영향. 대한한의학회지, 23(1), 83-91.

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Abstract

Objectives : In this studies, water extracts from Coix lachryma-jobi Linn var. mayuen Stapf. was investigated in their effects on insulin-like action and glucose uptake in 3T3-L1 cells.Methods : We examined the effects of insulin-like action on the differentiation of 3T3-L1 fibroblasts. The Coicis Semen was treated with hot water and the extract was freeze-dried. The hot water extract was chromatographed on nonionic polymer resin(Amberlite XAD-4, Sigma) with distilled water (Fr. 1), 20% (Fr. 2), 40% (Fr. 3), 60% (Fr. 4), 80% (Fr. 5), and 100% EtOH (Fr. 6), successively.Results : Total extract of Coicis Semen was fractionated into 0 to 100% MeOH with Amberlite XDA-4 column. Treatment of cells with 10 ㎍/㎖ of total extracts of Coicis Semen significantly increased the differentiation (p<0.05). At 1 ㎍/㎖ level of insulin, the differentiation was accelerated (p<0.01). The effect of extracts plus insulin on the differentiation was greater than that of insulin alone. In 3T3-L1 adipocytes, glucose uptake was higher by 2.7 times with 5 uM of total extract in low dosage of insulin (3 ng/㎖) than without total extract. 5 and 50 uM of water and 40% MeOH fractions increased glucose uptake by 3.5 times in 3T3-L1 adipocytes (p<0.001). Conclusions : Coicis Semen contains the compounds which play a role of insulin-like action and insulin sensitizer.

keywords: 3T3-L1 adipocytes, insulin-like action, insulin sensitizer, 3T3-L1 adipocytes, insulin-like action, insulin sensitizer

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薏苡仁이 3T3-L1 Adipocytes에서 인슐린성 작용과 인슐린 민감성에 미치는 영향

Effects of Insulin-like Action and Insulin Sensitizing on 3T3-L1 Adipocytes from Coicis Semen

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