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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

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樺皮의 抗炎 效果에 關한 硏究

Anti-inflammatory effects of an ethanolic extract from Betula Platyphylla

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2006, v.27 no.1, pp.184-195
유미현 (경희대학교)
박은경 (경희대학교)
김영훈 (경희대학교)
이연아 (경희대학교)
이상훈 (경희대학교)
양형인 (경희대학교)
홍승재 (포천중문의과대학)
백용현 (경희대학교)
박동석 (경희대학교)
한정수 (경희대학교)
유명철 (경희대학교)
김경수 (경희대학교)
유미현, 박은경, 김영훈, 이연아, 이상훈, 양형인, 홍승재, 백용현, 박동석, 한정수, 유명철, & 김경수. (2006). 樺皮의 抗炎 效果에 關한 硏究. 대한한의학회지, 27(1), 184-195.
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Abstract

Objectives : Betula Platyphylla (BP) is a traditional analgesic, anti-fungal, anti-inflammatory herb used in Chinese medicine. However, no information is available to explain its action. In this study, we investigated the anti-inflammatory effects of BP to elucidate the molecular pharmacological activity in the ethanol extract of BP(BPE).Methods : We performed MTS assay in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages with BPE. Nitrite was measured by Griess assay, prostaglandin E2 (PGE2) by enzyme-linked immunosorbent assay (ELISA) in LPS induced RAW264.7 macrophages with BPE. Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) were determined by Western blot. Activation of nuclear factor-kappaB (NF-κB) was measured by electrophoretic mobility shift assay (EMSA).Results : BPE significantly suppressed production of nitric oxide (NO) and PGE2 in LPS-induced RAW264.7 macrophages in a dose-dependent manner. The maximal inhibition rate of NO and PGE2 production by BPE was ca. 88.8% and 93% at the concentration of 100 ㎍/㎖ (non-cytotoxic concentration), respectively. BPE also decreased iNOS protein and COX-2 protein in LPS-induced RAW264.7 macrophages. EMSA demonstrated that BPE inhibited the DNA binding activity of the NF-κB.Conclusions : These results suggest that BPE inhibits NF-κB-mediated gene expression and downregulates inflammatory mediator production in RAW264.7 macrophages.

keywords
Betula Platyphylla, anti-inflammatory effect, Betula Platyphylla, anti-inflammatory effect


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