- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Inhibitory Effect of Rosmarinic acid Extrcted from Euonymus Alatus on Cyclooxygenase-2
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.5, pp.111-117
(2008). Inhibitory Effect of Rosmarinic acid Extrcted from Euonymus Alatus on Cyclooxygenase-2. Journal of Korean Medicine, 29(5), 111-117.
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Abstract
Objectives and methods:Previous mechanistic studies suggest the cyclooxygenase-2 (COX-2) inhibitors represent the good candidates against tumor progression. MeOH extract of the stem barks of Euonymus alatus induced the strong inhibition of COX-2. A phenolic compound responsible for the anti- COX-2 known to involve in tumor adhesion and invasion has been studied through the methanol extracts. The compound, rosmarinic acid (ROS-A) was an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid. ROS-A showed a strong inhibitory effect of COX-2 activity in a concentration-dependent manner. Then we have measured the IL-1β, IL-6 and TNF-α production related the immune regulation, induction of inflammatory related genes. Results and Conclusions:Hep3B cells produce proinflammatory cytokines of IL-1β, IL-6 and TNF-α while ROS A inhibited the cytokines production. Since IL-1β, IL-6 and TNF-α need the transcription factors such as nuclear factor-κB (NF-κB) and activated protein-1 (AP-1), we measured the transcription factors. ROS-A inhibited the activation of p65, p50, c-Rel subunits of NF-κB and AP-1 transcription factors. These findings indicate that ROS A from the stem bark of E. alatus inhibits proliferation in metastatic cancer cells. It was suggested that stem barks of E. alatus could be suitable for anti-cancer drugs.
- keywords
- Euonymus alatus, cyclooxygenase-2, Rosmarinic acid, transcription factor, cytokines
Reference
1
1. Osakabe N, Yasuda A, Natsume M, Yoshikawa T. Rosmarinic acid inhibits epidermal inflammatory responses: anticarcinogenic effect of Perilla frutescens extract in the murine two-stage skin model. Carcinogenesis. 2004 Apr;25(4):549-57.
2
2. Kitanaka S, Takido M, Mizoue K, Nakaike S. 1996. Cytotoxic cardenolides from woods of Euonymus alata. Chemical and pharmaceutical bulletin (Tokyo) 44, 615-617.
3
3. Lee H, Kim HK, Ha TY. 1993. Antitumor effect of winged Euonymus against chemically induced and malignant cell implanted-tumors in mice. Korean Journal of Immunology 15, 243-253.
4
4. Buckingham J. 1984. Dictionary of Organic compounds. New York, NY. Chapman and Hall, Cha BY, Park CJ, Lee DG, Lee YC, Kim DW, Kim JD, Seo WG, Moon SK, Kim CH, 2003. Inhibitory effect of methanol extract from Euonymus alatus on matrix metalloproteinase-9. Journal of Ethnopharmacology 85, 163-167.
5
5. Chung TW, Lee YC, Kim CH. Hepatitis B viral HBx induces matrix metalloproteinase-9 gene expression through activation of ERK and PI-3K/ AKT pathways: involvement of invasive potential. FASEB Journal. 2004;18: 1123-1125.
6
6. Chung TW, Moon SK, Chang YC, Ko JH, Lee YC, Cho G, Kim SH, Kim CH, 2004b. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. FASEB Journal 18(14), 1670-1681. 117
7
7. Park WH, Kim SH, Kim CH. 2005. A new matrix metalloproteinase-9 inhibitor 3,4-dihydroxycinnamic acid (caffeic acid) from methanol extract of Euonymus alatus: isolation and structure determination. Toxicology 207, 383-390.
8
8. Pradeep CR and G. Kuttan, 2004; Karin M, Delhase M. The I kappa B kinase (IKK) and NF-nB: key elements of proinflammatory signalling. Semin Immunol. 2000;12:85-98.
9
9. Shen Z, Peedikayil J, Olson GK, Siebert PD, Fang Y. Multiple transcription factor profiling by enzyme-linked immunoassay. BioTechniques 2002; 32:1168-77.
10
10. Parejo I, Caprai E, Bastida J, Viladomat F, Jauregui O, Codina C. 2004. Investigation of Lepechinia graveolens for its antioxidant activity and phenolic composition. Journal of Ethnopharmacology 94(1), 175-184.
11
11. Petersen M. and Simmonds, M.S.J., 2003. Rosmarinic acid. Phytochemistry 62, 121-125.
12
12. Parnham and Kesselring, 1985. M.J. Parnham and K. Kesselring. Rosmarinic acid. Drugs of the Future 10 (1985), 756-757.
13
13. D’Amelio, 1999. F.S. D’Amelio, Botanicals, A Phytocosmetic Desk Reference, CRC Press, London (1999) pp. 361.
14
14. Siebenlist K, Franzoso G, Brown K. Structure regulation and function of NF-nB. Annu Rev Cell Biol 1994;10:405-455.
15
15. Neurath MF, Becker C, Barbulescu K. Role of NF-nB in immune and inflammatory response in the gut. Gut 1998;439:856-860.
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