- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Immunohistochemical Study on the Hair Growth Promoting Effect of Yonnyuniksoogobon-dan
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.5, pp.77-89
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(2008). Immunohistochemical Study on the Hair Growth Promoting Effect of Yonnyuniksoogobon-dan. Journal of Korean Medicine, 29(5), 77-89.
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Abstract
Objectives:This study evaluates the hair growth promoting effect of Yonnyuniksoogobon-dan on shaved C57BL6 mice. Methods:Yonnyuniksoogobon-dan was administered orally (Group I) and both orally and by skin application (Group II) once a day for 12 days. The experimental groups were compared to Control, which was orally administered physiological saline solution. Hair regrowth was photographically and histologically determined during the experim- ental period. The levels of hair growth cycle related factors (EGF, TGF-β1) and vascular factors (VEGF, iNOS) were also determined by immunohistochemistry. On gross observation of hair growth, both Group I and Group II shaved C57BL6 mice showed accelerated hair regrowth. Results:The hair regrowth index of Group I increased significantly from 7 days (P<0.05) to 12 days (P<0.01) after shaving and that of Group II was significantly higher at 12 days (P<0.05). On histological observation, both Group I and Group II demonstrated histological improvement and increases in the number and diameter of the hair follicles. EGF expression on the root sheath of hair follicles was up-regulated in both Group I and II. TGF-β1 expression on the root sheath of hair follicles was unchanged in both Group I and II. VEGF expression in the tissues surrounding hair follicles was up-regulated in both groups. iNOS expression in the tissues surrounding hair follicles was down-regulated in both groups. Conclusions:These results suggest that Yonnyuniksoogobon-dan promotes hair growth and this effect is related to up-regulation of EGF and VEFG expression and down-regulation of TGF-β1 and iNOS expression on hair roots.
- keywords
- Yonnyuniksoogobon-dan hair growth, C57BL6 mice, EGF, TGF-β1, VEGF, iNOS, Yonnyuniksoogobon-dan hair growth, C57BL6 mice, EGF, TGF-β1, VEGF, iNOS
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