- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Studies on the Anti-cancer Activity of Chaga Mushroom Extract
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.4, pp.1-12
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(2009). Studies on the Anti-cancer Activity of Chaga Mushroom Extract. Journal of Korean Medicine, 30(4), 1-12.
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Abstract
Objectives:This study was to investigate the anti-oxidation and anti-cancer activity of Chaga mushroom extract. Extraction condition optimization and beta-glucan analysis and anti-cancer activity tests were also done. Methods:Optimum extraction conditions for Chaga mushroom extract were at a temperature of 90℃ and 2hrs with 10 times of water. Extraction yield and economics were best under these conditions. Results:Anti-oxidation activity was the highest with the fraction of 100,000 MWCO and IC50 value was 13㎍/㎖, and this value was comparable to that of vitamin E, alpha-tocopherol. Among the fractions from various organic solvents, ethyl acetate fraction showed the highest anti-oxidation activity with IC50 value of 7㎍/㎖. For anti-cancer activity, chloroform fraction showed little anti-cancer activity and ethyl acetate fraction showed the best anti-cancer activity with IC50 1.5㎍/㎖ for stomach cancer cells. Anti-cancer activities for different molecular weight fractions were the best in the fraction of molecular weight less than 100,000Da, and IC50 values for stomach cancer cells and liver cancer cells were 1.7 and 1.4㎍/㎖, respectively. Conclusions:From these results, we can conclude that the extract of Chaga mushroom could be a good source for functional food and natural anti-cancer medicine .
- keywords
- Inonotus obliquus, anti-oxidation, anti-cancer, beta-glucan
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