- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
The Effect of Trans-cinnamaldehyde on the Gene Expression of Lipopolysaccharide-stimulated BV-2 Cells Using Microarray Analysis
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.4, pp.13-27
&
(2009). The Effect of Trans-cinnamaldehyde on the Gene Expression of Lipopolysaccharide-stimulated BV-2 Cells Using Microarray Analysis. Journal of Korean Medicine, 30(4), 13-27.
- Downloaded
- Viewed
Abstract
Objectives:Trans-cinnamaldehyde (TCA) is the main component of Cinnamomi Ramulus and it has been reported that TCA inhibits inflammatory responses in various cell types. Inflammation-mediated neurological disorders induce the activation of macrophages such as microglia in brain, and these activated macrophages release various inflammation-related molecules, which can be neurotoxic if overproduced. In this study, we evaluated gene expression profiles using gene chip microarrays in lipopolysaccharide (LPS)-stimulated BV-2 cells to investigate the anti- inflammatory effect of TCA on inflammatory responses in brain microglia. Methods:A negative control group was cultured in normal medium and a positive control group was stimulated with 1 ㎍/㎖ LPS in the absence of TCA. TCA group was pretreated with 10 ㎍/㎖ TCA before 1 ㎍/㎖ LPS stimulation. The oligonucleotide microarray analysis was performed to obtain the expression profiles of 28,853 genes using gene chip mouse gene 1.0 ST array in this study. Results:In positive control group, 1522 probe sets were up-regulated in the condition of the cutoff value of 1.5-fold change and 341 genes with Unigene ID were retrieved. In TCA group, 590 probe sets were down-regulated from among 1522 probe sets and 33 genes with Unigene ID were retrieved, which included 6 inflammation-related genes. We found out that Id3 gene is associated with transforming growth factor-β (TGF-β) signaling pathway and Klra8 gene is related to natural killer cell-mediated cytotoxicity pathway. Conclusions:The results mean that TCA inhibits inflammatory responses through down-regulating the expressions of inflammation-related genes in LPS-stimulated BV-2 cells.
- keywords
- trans-cinnamaldehyde, lipopolysaccharide, microglia, BV-2, microarray
Reference
1
KFDA (Korea Food & Drug Administration). The Korean Herbal Phamacopoeia. 8th ed. Seoul: Yakup Daily. 2002:1241-1243.
2
Park HJ, Lee JS, Lee JD, Kim NJ, Pyo JH, Kang JM, et al.. The anti-inflammatory effect of Cinnamomi Ramulus. J Korean Oriental Med. 2005; 26(2):140-151.
3
Lee HS, Kim BS, Kim MK. Suppression effect of Cinnamomum cassia derived component on nitric oxide synthase. J Agric Food Chem 2002;50:7700-7703.
4
Guo JY, Huo HR, Zhao BS, Liu HB, Li LF, Ma YY, et al.. Cinnamaldehyde reduces IL-1betainduced cyclooxygenase-2 activity in rat cerebral microvascular endothelial cells. Eur J Pharmacol. 2006; 537(1-3):174-180.
5
Chao LK, Hua KF, Hsu HY, Cheng SS, Lin IF, Chen CJ, et al.. Cinnamaldehyde inhibits proinflammatory cytokines secretion from monocytes/ macrophages through suppression of intracellular signaling. Food Chem Toxicol. 2008; 46(1):220-231.
6
Lundberg IE. The role of cytokines, chemokines and adhesion molecules in the pathogenesis of idiopathic inflammatory myopathies. Current Rheumatology Report. 2000;2:216-224.
7
Murabe Y, Sano Y. Morphological studies on neuralgia. . Postnatal development of microglia cells. Cell Tissue Res. 1982; 225: 469-485.
8
Gonzalez-Scarano F, Baultuch G. Microglia as mediators of inflammatory and degenerative disease. Annual Rev Neurosci. 1999;34:307-321.
9
Orr CF, Rowe DB, Haliday GM. An inflammatory review of Parkinson’s disease. Progress in Neurobiology. 2002;68:325-340.
10
Norton WT, Aquino DA, Hozymi I, Chiu FC, Brosnan CF. Quantitive aspects of reactive gliosis: a review. Neurochem. Res. 1992;17:877-885.
11
GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual Version 4; Copyright 2007, Affymetrix, Inc.
12
Kim DH, Kim CH, Kim MS, Kim JY, Jung KJ, Chung JH, et al.. Suppression of age-related inflammatory NF-κB activation by cinnamaldehyde. Biogerontology 2007;8:545-554.
13
Meme W, Calvo CF, Froger N, Ezan P, Amigou E, Koulakoff A, et al.. Proinflammatory cytokines released from microglia inhibit gap junctions in astrocyte: potentiation by beta-amyloid. FASEB J. 2006;20:494-496.
14
Lue LF, Rydel R, Brigham EF, Yang LB, Hampel H, Murphy GM Jr, et al.. Infalmmatory repertoire of Alzheimer’s disease and nondemented elderly microglia in vitro. Glia. 2001; 35: 72-79.
15
Moilanen E, Whittle B, Moncada S. Nitric oxide as a factor in inflammation. In: Gallin JI, Snyderman R, Editors, Inflammation: basic principles and clinical corralates. Williams and Wilkins, Philadelphia. 1999;787-800.
16
Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol Rev. 1991;43:109-142.
17
Campbell A. Inflammation, neurodegenerative diseases, and environmental exposures. Ann. N.Y. Acad. Sci. 2004;1035:117-132.
18
Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cellular Signaling. 2001;13:85-94.
19
Stuechr DJ. Structure-function aspects in the nitric oxide synthases. Annu. Rev. Pharmacol. Toxicol. 1997;37:339-359.
20
Meda L, Cassatella MA, Szendrei GI, Otvos L, Baron P, Villalba M, et al.. Activation of micr-oglial cell by β-amyloid protein and interferon-γ. Nature. 1995;374:647-650.
21
Miyasaka N, Hirata Y. Nitric oxide and inflammatory arthritides. Life Sci. 1997;61(21):2073-2081.
22
Lucas SM, Rothwell NJ, Gibson RM. The role of inflammation in CNS injury and disease. British Journal of Pharmacology. 2006;147:232-240.
23
Sedgwick JD, Riminton DS, Cyster JG, Korner H. Tumor necrosis factor: a master-regulator leukocyte movement. Immunol Today. 2000;21:110-113.
24
Hoozemans JJ, Veerhuis R, Janssen I, van Elk EJ, Rozemuller AJ, Eikelenboom P. The role of cyclo-oxygenase 1 and 2 activity in prostaglandin E (2) secretion by cultured human adult microglia: implications for Alzheimer’s disease. Brain Res. 2002;951:218-226.
25
Teismann P, Tieu K, Choi DK, Wu DC, Naini A, Hunot S, et al.. Cyclooxygenase-2 is instrumental in Parkinson’s disease neurodegeneration. Proc Natl Acad Sci USA. 2003;100(9):5473-5478.
26
Leib SL, Leppert D, Clements J, Tauber MG. Matrix metalloproteinases contribute to brain damage in experimental pneumococcal meningitis. Infect Immun. 2000;68:615-620.
27
Kieseier BC, Schneider C, Clements JM, Gearing AJ, Gold R, Toyka KV, et al.. Expression of specific matrix metalloproteinases in inflammatory myopathies. Brain. 2001;124:341-351.
28
Jiang Z, Johnson HJ, Nie H, Qin J, Bird TA, Li X. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)- IRAK-tumor necrosis factor receptor- associated factor 6 (TRAF6) complex. J Biol Chem. 2003;278(13):10952-10956.
29
Lyons RM, Moses HL. Transforming growth factors and the regulation of cell proliferation. Eur J Biochem. 1990;187:461-473.
30
Howe HL, Wingo PA, Thun MJ, Ries LA, Rosenberg HM, Feigal EG, et al.. Annual report to the nation on the status of cancer (1973 through 1998), featuring cancers with recent increasing trends. J Natl Cancer Inst. 2001;93:824-842.
31
Massari ME, Murre C. Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms. Mol Cell Biol. 2000;20:429-440.
32
Norton JD. ID helix-loop-helix proteins in cell growth, differentiation and tumorigenesis. J Cell Sci. 2000;113:3897-3905.
33
Springhorn JP, Singh K, Kelly RA, Smith TW. Posttranscriptional regulation of Id1 activity in cardiac muscle. Alternative splicing of novel Id1 transcript permits homodimerization. J Biol Chem. 1994;269:5132-5136.
34
Martin M, Lefaix J, Delanian S. TGF-β1 and radiation fibrosis : a master switch and a specific therapeutic target. International Journal of Radiation Oncology, Bio and Physics. 2000;47(2):277-290.
35
Yue J, Mulder KM. Transforming growth factor-β signal transduction in epithelial cells. Pharmacology & Therapeutics. 2001;91:1-34.
36
Kowanetz M, Valcourt U, Bergström R, Heldin CH, Moustakas A. Id2 and Id3 define the potency of cell proliferation and differentiation responses to transforming growth factor beta and bone morphogenetic protein. Mol Cell Biol. 2004;24(10):4241-4254.
37
Kim WK, Hwang SY, Oh ES, Piao HZ, Kim KW, Han IO. TGF-beta1 represses activation and resultant death of microglia via inhibition of phosphatidylinositol 3-kinase activity. J Immunol. 2004;172:7015-7023.
38
Wyss-Coray T. TGF-Beta pathway as a potential target in neurodegeneration and Alzheimer’s. Curr Alzheimer Res. 2006;3(3):191-195.
39
Kleinert H, Euchenholer C, Ihrig-Biedert I, Forstermann U. Glucocorticoids inhibit the induction of nitric oxide synthase by Ⅱ downregulating cytokine -induced activity of transcription factor nuclear factor-κB. Mol Pharmacol. 1996;49:15-21.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Studies on the Anti-cancer Activity of Chaga Mushroom Extract
- The Effect of Trans-cinnamaldehyde on the Gene Expression of Lipopolysaccharide-stimulated BV-2 Cells Using Microarray Analysis
- Need for Development of Oriental Medicine-derived Systemic Treatments against Chronic Fatigue-associated Symptoms
- Consideration on the Paste Preparation Based on Entries from the Annals of the Chosun Dynasty
- Collecting Method and Implementation of Search Management System for R&D Projects in Korean Medicine Field
- Study on the Sasang Constitutional Difference between Married Men & Women
- A Study on Korean Oriental Medical Doctors’ Use of Uninsured Herbal Extracts and How to Promote the Insurance Coverage of Such Herbal Extracts
- Neuroprotective Effect of PD-1 Extract in MPTP-lesioned Mouse Model of Parkinson’s Disease
- Effects of the Houttuyniae Herba Extract on the Membranous Nephropathy induced by Cationic Bovine Serum Albumin in Mice
- Concentration of Heavy Metals, Residual Pesticides and Sulfur Dioxide before/after a Decoction - In Prescription consist of Sipjeondaebo-tang -
- Survey on Revision and Complements for the Current Curriculum of Herbology
- The Anticancer Effects and Drug Metabolic Enzyme Change by Intraperitoneal Injection of Agrimonia Pilosa Ledeb
- The Cause of Jeong-Jo’s Death Based on The Annals of the Chosun Dynasty (朝鮮王朝實錄), Seungjeongwon Ilgi (承政院日記)
- A Case Report of Symptomatic Character and Diagnosis of Fibromyalgia
- Oriental Medical Therapy for Sudden Sensorineural Hearing Loss
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.