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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

The Anticancer Effects and Drug Metabolic Enzyme Change by Intraperitoneal Injection of Agrimonia Pilosa Ledeb

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.4, pp.129-141










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Abstract

Objective:This study was to investigate the anti-tumor effect, safety, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB (APL) in female C57B/L mouse tumor (in vivo). Method:First, to evaluate the antitumor activity of APL, we divided the mice into four groups: normal, control, APL50 (50㎎/㎏), and APL100 (100㎎/㎏). LLC-obtained American Type Culture Collection was used. LLC had been inoculated to induce tumors. To measure the anti-tumor effect of APL, we calibrated tumor size and weight. To analyze the mechanism of anti-tumor in APL, we used western blotting and to observe metabolizing enzyme in APL we used to real-time PCR. Result:APL50 and APL100 significantly inhibited tumor growth from 12 days after medicine injected. APL did not induce caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it decreased 41% and 71% in CYP2D22 and CYP3A11, respectively. Conclusion:These results suggest that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be used carefully with other drugs related with CYP2D22 and CYP3A11.

keywords
APL, anti-tumor, PCR, western blot, LLC


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