Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Antioxidative Activities of Wen-pi-tang-Hab-Wu-ling-san (WHW®) in vitro

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.5, pp.146-156


  • Downloaded
  • Viewed

Abstract

Objectives:The objective of this study was to investigate the antioxidant effects of manufactured Wen-pi-tang-Hab- Wu-ling-san (WHW®) in vitro. Methods:WHW® was prepared by the pilot manufacture of WHW water extract from a GMP system appointed company. Antioxidative activities were determined by in vitro tests as follows: the scavenging activities of oxygen free radicals including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion, hydrogen peroxide and nitric oxide radicals, as well as ferrous ion chelating capacity and Trolox equivalent antioxidant capacity (TEAC). Results:WHW® significantly scavenged oxygen free radicals such as DPPH (IC50=115.28 ± 0.25 ㎍/㎖), superoxide anion (IC50=8.56 ± 0.08 ㎍/㎖), hydrogen peroxide (IC50=240.36 ± 3.41 ㎍/㎖) and nitric oxide (IC50=162.28 ± 0.21 ㎍/㎖) radicals. WHW® also showed ferrous ion chelating activity (IC50=543.19 ± 4.85 ㎍/㎖) and Trolox equivalent effects (IC50=45.311 ㎍/㎖) in TEAC and ORAC assay, respectively. Conclusion:This study demonstrates that WHW® has strong antioxidative properties through free radical scavenging activity. These data suggest that WHW® be used as an antioxidant agent.

keywords
Traditional Korean medicine, Wen-pi-tang-Hab-Wu-ling-san, antioxidation, free radicals


Reference

1

Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann N Y Acad Sci 2000;899:136-47.

2

Kim SK. Evaluation of antioxidant activity. Safe Food 2008;4:35-40.

3

Cross EE, B Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, et al. Oxygen radicals and human disease (clinical conference). Ann. Intern. Med. 1987;107:526-45.

4

Adelson R, Saul RL, Ames BN. Oxidative damage to DNA: Relation to species metabolic and life span. Proc Natl Acad Sci 1988;85: 2706-8.

5

Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India. 2004:794-804. Review.

6

Nelson L, Perrone J. Herbal and alternative medicine. Emerg Med Clin North Am. 2000; 18:709 22.

7

Jang DM. Practical sinbyeonghak of Chinese Medicine. Beijing;Chinise Medicine Publication. 1990 pp. 344-46.

8

Jang GB. Gyeongakjeonsu. Seoul;Dae Sung Printing. 1988 p. 467.

9

Gang GM, Pomyeonghye. Simplicity bangje dictionary. Beijing;Chinise Medicine Publication. 1989. pp. 173, 887, 1146.

10

Nanjing Institute of Chinese medicine. Chinese medicine bangje dictionary;Beijing. People’s Medical Publishing House. 1997. pp. 10844-47.

11

Zhongshan Medical Institute. Junguibangjeseongang. Guangdong;Guangdong Science & Technology Press. 1981. pp. 84-9.

12

Sun S. Qian-Jin-Yi- Fang. Taebuk;Jipmunseoguk. 1989. p. 238

13

Takizawa Y, Morita T, Fujitsuka N, Kido A. Shindou S. Tomisawa H, et al. Pharmacokinetics of TJ-8117(Onpi-to), a drug for renal failure (II): Effects of food, repeated administration and renal failure to plasma concentration of [3H]-(-) epicatechin 3-O-gallate in rats. Eur J Drug metabolism and pharmacokinetics, 2005;30:175-80.

14

Hattori T, Fujitsuka N, Kurogi A, Shindo S. Effect of Onpi-to on TGF-beta 1 in rats with 5/6 nephrectomized chronic renal failure, Nippon Jinzo, Gakkai Shi. 1996;38:475-83.

15

Yokozawa T, Satoh A, Nakagawa T, Yamabe N. Attenuating effects of wen-pi-tang treatment in rats with diabetic nephropathy. Am J Chin Med. 2006;34:307-21.

16

Kim KT, Doo HK. Effects of Yukmijihwangang on the Alloxan-induced Diabetic and Renal Damage Rats. K.H.M. 1988;4:280-97.

17

Hwang WD. A Report on Clinical application of Chenxiang about Chronic Renal Failure. Korean Oriental Internal Medicine. 2004;25:368-78.

18

Ahn YM, Ahn SY, Doo HK. The immunoregulatory effects of Onbi-tang and Dangguijakyaksan in minimal change nephrotic syndrome. J Korean Oriental Med. 2000;21:20-8.

19

Doo HK, Oh SD, Ahn SY, Cho DH. Effects of Akebia quinata DENCE and Stephania tetrandra S. MOORE on Rats with Acute Renal Failure Induced by Gentamicin Sulfate. K.H.M. 1994;10:13-25.

20

Lee SI, Kim HJ, Baek MC, Park KM, Park Y, Yoon CH, et al. Wen-pi-tang-Hab-Wu-ling-san, an oriental herbal prescription, attenuates epithelialmesenchymal transdifferentiation stimulated by TGF-beta1 in kidney cells. Phytother Res. 2007;21:548-53.

21

Seok YM, Kim J, Park MJ, Boo YC, Park YK, Park KM. Wen-pi-tang-Hab-Wu-ling-san attenuates kidney fibrosis induced by ischemia/reperfusion in mice. Phytother Res. 2008;22:1057-63.

22

Seok YM, Kim J, Choi KC, Yoon CH, Boo YC, Park Y, et al. Wen-pi-tang-Hab-Wu-ling-san attenuates kidney ischemia/reperfusion injury in mice. A role for antioxidant enzymes and heatshock proteins. J Ethnopharmacol. 2007;112:333-40.

23

Jung HW, Yoon CH, Kim YH, Boo YC, Park KM, Park YK. Wen-Pi-Tang-Hab-Wu-Ling-San extract inhibits the release of inflammatory mediators from LPS-stimulated mouse macrophages. J Ethnopharmacol. 2007;114:439-45.

24

Gyamfi MA, Yonamine M, Aniya Y. Freeradical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally induced liver injuries. General Pharmacology 1999; 32:661-67.

25

Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxide anions. Biochemical Pharmacology 1988;36:317-22

26

Marcocci PL, Sckaki A, Albert GM. Antioxidant action of Ginkgobiloba extracts EGP761. Methods in Enzymology 1994;234:462-75.

27

Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agriculture and Food Chemistry 1990;38:674-77.

28

Schlesier K, Harwat M, Böhm V. and Bitsch, R. Assessment of antioxidant activity by using different in vitro methods. Free Radical Research 2002;36:177-87.

29

Cao G, Verdon CP, Wu AHB, Wang H, Prior RL. Automated assay of oxygen radical absorbance capacity with the COBAS FARA II. Clinical Chemistry 1995;41:1738-44.

30

Kuramoto T. Development and application of food materials from plant extracts such as SODUp to date Food Processing 1992;27:22-3.

31

Prior RL, Wu X, Chaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem. 2005;52:4290- 302.

32

Kim SJ, Kweon DH, Lee JH. Investigation of antioxidative activity and stability of ethanol extracts of Licorice root (Glycyrrhiza glabra). Korean J Food Sci Technol 2006;38;584-8.

33

Yang SA, Im NK, Lee IS. Effects of Methanolic Extract from Salvia miltiorrhiza Bunge on in vitro Antithrombotic and Antioxidative Activities. Korean J Food Sci Technol 2007;39:83-7.

34

Kim CJ, Suh HJ. Antioxidant Activities of Rhubarb Extracts Containing Phenolic Compounds. Korean J Food Culture. 2005;20:77-85.

35

Kim DG, Son DH, Choi UK, Cho YS, Kim SM.The Antioxidant Ability and Nitrite Scavenging Ability of Poria cocos. J Korean Soc Food Sci Nitr 2002;31:1097-101.

36

Nam HS, Cho CS, Kim CJ. A Study on the Healing Mechanism of Herbal Medicine, Oryoungsan. Oriental Medicine Journal of Daejeon University. 2001;10:157-66.

37

Kang YH, Kwon OS,Choi HS, Lee JH. Effect of the Oryungsan on the injured liver in CCl4-treated rats. Oriental Medicine Journal of Dongguk University. 1996;5:131-47.

38

Cho DH, Doo HK, Kim IS, Son SY, Jung YH, Han YH, et al. Effects of Gamioryeongsan, Gamiyukmijihwangtang and Vinegar on Rats with Acute Renal Failure Induced by Gentamicin Sulfate. K.H.M. 1991;7:287-312.

39

Lee JJ, Lee TW, Chang HH, Kim HJ, Ihm CG, Kim MJ. Inhibitory Effects of Siryungtang and Onbitang on Oxidation of Low Density Lipoprotein. The Korean J Nephrology 2001;20:478-85.

  • Downloaded
  • Viewed
  • 0KCI Citations
  • 0WOS Citations

Other articles from this issue

Recommanded Articles

상단으로 이동

Journal of Korean Medicine