- P-ISSN 1010-0695
- E-ISSN 2288-3339
Objectives:The objective of this study was to investigate the antioxidant effects of manufactured Wen-pi-tang-Hab- Wu-ling-san (WHW®) in vitro. Methods:WHW® was prepared by the pilot manufacture of WHW water extract from a GMP system appointed company. Antioxidative activities were determined by in vitro tests as follows: the scavenging activities of oxygen free radicals including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion, hydrogen peroxide and nitric oxide radicals, as well as ferrous ion chelating capacity and Trolox equivalent antioxidant capacity (TEAC). Results:WHW® significantly scavenged oxygen free radicals such as DPPH (IC50=115.28 ± 0.25 ㎍/㎖), superoxide anion (IC50=8.56 ± 0.08 ㎍/㎖), hydrogen peroxide (IC50=240.36 ± 3.41 ㎍/㎖) and nitric oxide (IC50=162.28 ± 0.21 ㎍/㎖) radicals. WHW® also showed ferrous ion chelating activity (IC50=543.19 ± 4.85 ㎍/㎖) and Trolox equivalent effects (IC50=45.311 ㎍/㎖) in TEAC and ORAC assay, respectively. Conclusion:This study demonstrates that WHW® has strong antioxidative properties through free radical scavenging activity. These data suggest that WHW® be used as an antioxidant agent.
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