- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Broussonetia kazinoki Siebold stimulates immune response in ovalbumin-immunized mice
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2011, v.32 no.3, pp.10-17
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Jeonju Biomaterials Institute)
(ChunYang Paper Co., Ltd.)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Jeonju Biomaterials Institute)
(ChunYang Paper Co., Ltd.)
Dayoung,
J.
, Hyekyung,
H.
, Hoyoung,
L.
, Jin-Ah,
L.
, Seung-Il,
J.
, Young-Jae,
C.
, &
(2011). Broussonetia kazinoki Siebold stimulates immune response in ovalbumin-immunized mice. Journal of Korean Medicine, 32(3), 10-17.
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Abstract
Objective: To evaluate the immune-stimulatory potential of extracts of Broussonetia kazinoki Siebold (BK) on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Material and Methods: C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 μg/200 μg) on days 1, 8, and 15. BK (100, 300 or 1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Further, the effects of BK on expression of cytokine mRNA in OVA-immunized mice splenocytes were evaluated by RT-PCR analysis. Results: BK significantly enhanced OVA-, LPS-, and Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). BK also significantly enhanced total IgM and OVA-specific IgG1 levels in plasma compared with the OVA control group. Moreover, BK up-regulated significantly the expression of mRNA level of IL-2 and IFN-γ in splenocytes. Conclusions: BK has immune-stimulating activity in an OVA-immunized mouse model system, enhancing the Th1 immune response. BK showed no cytotoxicity in this system, suggesting that BK may be a safe and effective adjuvant in humans.
- keywords
- Broussonetia kazinoki Siebold, OVA, splenocyte proliferation, antibody, adjuvant, immune response
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