- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Evaluation of the Immune-Stimulating Activity of Samul-tang, a Traditional Korean Herbal Medicine, Standardized by HPLC-PDA
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2011, v.32 no.3, pp.25-34
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
(Korea Institute of Oriental Medicine)
Hyekyung,
H.
, Dayoung,
J.
, Ho,
Y.
L.
, &
(2011). Evaluation of the Immune-Stimulating Activity of Samul-tang, a Traditional Korean Herbal Medicine, Standardized by HPLC-PDA. Journal of Korean Medicine, 32(3), 25-34.
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Abstract
Objectives: We performed simultaneous determination of five constituents by HPLC in Samul-tang (SMT). Additionally, we investigated the immune-stimulatory potential of SMT on specific cellular and humoral immune responses in ovalbumin (OVA)-immunized mice. Methods: Reverse-phase chromatography using a Gemini C18 column operating at 40°C, and photodiode array (PDA) detection at 190-400 nm, were used for quantification of the five components of SMT. Mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. C57BL/6 mice were immunized intraperitoneally with OVA/alum (100 μg/200 μg) on days 1, 8, and 15. The extract of SMT (1000 mg/kg) was given to mice orally for 21 days (from day 1 to day 21). At day 22, OVA-, lipopolysaccharide (LPS)- and concanavalin A (Con A)-stimulated splenocyte proliferation and OVA-specific and total antibodies were measured in plasma. Results: Calibration curves were acquired with r^2>0.9999, and the relative standard deviation (RSD, %) for intra- and inter-day precision were both less than 3.5%. The recovery was in the range of 95.69-115.12%, with an RSD less than 6.0%. The contents of five components in SMT were 1.08-15.30 mg/g. SMT significantly enhanced Con A-induced splenocyte proliferation in OVA-immunized mice (p<0.01). Also, SMT significantly enhanced OVA- specific IgG, IgG1 and total IgM levels in plasma compared with the OVA-immunized group. Conclusions: The established method will be applied for the quantification of major components and immune- stimulating activity in OVA-immunized mouse model of SMT.
- keywords
- Herbal medicine, HPLC-PDA, Samul-tang, simultaneous determination, immune-stimulating activity
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