Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Therapeutic Effects of Coptidis Rhizoma and Berberine in Streptozotocin-induced Diabetic Rats

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2011, v.32 no.6, pp.1-9
Kee-Tae Kweon (Division of Traditional Korean Medicine Industry)
Sang-young Ahn (Korea Institute of Oriental Medicine)
In-hye Ham (Kyung Hee University)

Ho-Young Choi (Kyung Hee University)
  • Downloaded
  • Viewed

Abstract

Objectives: We performed this study to compare the antidiabetic effects of Coptidis Rhizoma (CR) and its major component berberine with gliclazide. Materials and Methods: Diabetic rats induced by injection of streptozotocin (STZ) 55mg/kg were treated with CR 100, 200, 400mg/kg and berberine 100mg/kg. After rats were treated for 5 days, serum glucose, total cholesterol, triglyceride, BUN, creatinine and antioxidant levels were determined. Results: The cytotoxic effects of CR (0.1, 0.01, and 0.001mg/mL), berberine and gliclazide (0.1µM, 1µM, and 10µM) were tested in rat insulinoma (RIN) cells induced with 5mM STZ. The levels of fasting blood glucose, total cholesterol, triglyceride, BUN and creatinine of CR and berberine treated groups were reduced as much as that of gliclazide group in comparison to control groups, whereas total antioxidant levels increased. In vitro experiments showed that CR and berberine have a cytoprotective effect on RIN cells.

keywords
Coptidis rhizome, berberine, streptozotocin, gliclazide, diabetes


Reference

1

1. Li WL, Zheng HC, Bukuru J, Kimpe ND. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol. 2004; 92(1):1-21.

2

2 Cockram CS. Diabetes mellitus: perspective from the Asia-Pacific region. Diabetes Res Clin Pract. 2000; 50(2):S3-S7.

3

3 Heo J. Dongeuibogam. Seoul:Bubin publisher co. 1999:1341.

4

4 Choi YS, Lee YI, Lee SY. Effects of extracts of Coptis japonica on lipid metabolism in rats. Kor J Pharmacogn. 1996; 27(3):246-253.

5

5 Kim YJ, Lee MJ, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant activity of water-extract from Coptidis chinensis Franch. Journal of Life Science. 2000; 10(3):241-246.

6

6 Saltiel AR. New Perspectives into the Molecular Pathogenesis and Treatment of Type 2 Diabetes. Cell. 2001; 104:517-529.

7

7 Taskinen M. LDL-cholesterol, HDL-cholesterol or triglycerides which is the culprit? Diabetes Res Clin Pract. 2003; 61:S19-S26.

8

8 Zhou L, Yang Y, Wang X, Liu S, Shang W, Yuan G, et al. Berberine stimulates glucose transport through a mechanism distinct from insulin. Metabolism. 2007; 56(3):405-412.

9

9 Park JY, Lee KU, Kim CH, Kim HK, Hong SK, Park KS, et al. Past and current obesity in Koreans with non-insulin-dependent diabetes mellitus. Diabetes Res Clin Pract.1997; 35:49-56.

10

10. Bensky D, Gamble A. Chinese herbal medicine: Materia Medica. Revised Edition. Seattle: Eastland Press, Inc., 1993; 77-80.

11

11. Iizuka N, Miyamoto K, Okita K, Tangoku A, Hayashi H, Yosino Y, et al. Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Lett. 2000; 148:19-25.

12

12. Shigeta K, Ootaki K, Tatemoto H, Nakanishi K, Inada A, Muto N. Potentiation of Nerve Growth Factor-Induced Neurite Outgrowth in PC12 Cells by a Coptidis Rhizoma Extract and Protoberberine Alkaloids. Biosci Biotechnol Biochem. 2002; 66(11):2491-2492.

13

13. Kwon KB, Kim EK, Lim JG, Shin BC, Han SC, Song BK, et al. Protective effect of Coptidis Rhizoma on S-nitroso-N-acetylpenicillamine (SNAP)- induced apoptosis and necrosis in pancreatic RINm5F cells. Life Sciences. 2005; 76:917-929.

14

14. Kim EK, Kwon KB, Han MJ, Song MY, Lee JH, Lv N, et al. Coptidis rhizoma extract protects against cytokine-induced death of pancreatic β- cells through suppression of NF-κβ activation. Exp and Mol Med. 2007; 39(2):149-159.

15

15. Lin SS, Chung JG, Lin JP, Chuang JY, Chang WC, Wu JY, et al. Berberine inhibits arylamine N-acetyltransferase activity and gene expression in mouse leukemia L1210 cell. Phytomedicine. 2005; 12:351-358.

16

16. Mitani N, Murakami K, Yamamura T, Ikeda T, Saiki I. Inhibitory effect of berberine on the mediastinal lymph node metastasis produced by orthotopic implantation of Lewis lung carcinoma. Cancer Lett. 2001; 65:35-42.

17

17. Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H. Inhibition by berberine of cyclooxygenase- 2 transcriptional activity in human colon cancer cells. J Ethnopharmacol. 1999; 66:227-233.

18

18. Kuo CL, Chi CW, Liu TY. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer Lett. 2004; 203:127-137.

19

19. Tang LQ, Wei W, Chen LM, Liu S. Effects of berberine on diabetes induced by alloxan and a high-fat/high-cholesterol diet in rats. J Ethnopharmacol. 2006; 108:109-115.

20

20 Jia W, Gao W, Tang L. Antidiabetic herbal drugs officially approved in China. Phytother Res. 2003; 17:1127-1134.

21

21. Kar A, Choudhary BK, Bandyopadhyay NG. Comparative evaluation of hypoglycemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol. 2003; 84(1): 105-108.

22

22. Leng SH, Lu FE, Xu LJ. Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion. Acta Pharmacologica Sinica. 2004; 25(4):496-502.

23

23. Yin J, Hu R, Chen M, Tang J, Li F, Yang Y, et al. Effects of Berberine on Glucose Metabolism in vitro. Metabolism. 2002; 51(11):1439-1443.

24

24. Pan GY, Huang ZJ, Wang GJ, Fawcett JP, Liu XD, Zhao XC, et al. The antihyperglycaemic activity of berberine arises from a decrease of glucose absorption. Planta Med. 2003; 69(7): 632-636.

25

25. Saxena R, Madhu SV, Shukla R, Prabhu KM Gambhir JK. Postprandial hypertriglyceridemia and oxidative stress in patients of type 2 diabetes mellitus with macrovascular complication. Clinica Chimica Acta. 2006; 368(1-2):101-108.

26

26. Barakat HA, Vadlamudi S, MacLean PS, MacDonald KG, Pories WJ. Lipoprotein metabolism in noninsulin- dependent diabetes mellitus. Nutritional Biochemistry. 1996; 7:586-598.

27

27. Yokozawa T, Ishida A, Cho EJ, Nakagawa T. The effects of Coptidis Rhizoma extract on a hypercholesterolemic animal model. Phytomedicine. 2003; 1(10):17-22.

28

28. Phillips DI, Caddy S, Ilic V, Fielding BA, Frayn KN, Borthwick AC, et al. Intramuscular triglyceride and muscle insulin sensitivity: Evidence for a relationship in nondiabetic subjects. Metabolism. 1996; 45(8):947-950.

29

29. Koyama K, Chen G, Lee Y, Unger UH. Tissue triglycerides, insulin resistance, and insulin production: implication for hyperinsulinemia of obesity. Am j Physiol. 1997; 273:708-713.

30

30. Cho EJ, Yokozawa T, Rhee SH, Park KY. The role of Coptidis Rhizoma extract in a renal ischemia-reperfusion model. Phytomedicine. 2004; 11:576-584.

31

31. Kaneto H, Kajimoto Y, Miyagawa J, Matsuoka T, Fujitani Y, Umayahara Y, et al. Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes. 1999; 48:2398-2406.

32

32. Anderson RA, Evans ML, Ellis GR, Graham J, Morris K, Jackson SK, et al. The relationship between pots-prandial lipaemia, endothelial function and oxidative stress in healthy individuals and patients with type 2 diabetes. Atherosclerosis. 2001; 154:475-483.

33

33. Cho EJ, Yokozawa T, Kim HY, Shibahara N, Park C. Rosa rugosa attenuates diabetic oxidative stress in rats with streptozotocin-induced diabetes. Am J Chin Med. 2004; 32(4):487-496.

34

34. Baynes JW, Thorpe SR. Role of oxidative stress in diabetic complication, a new perspective on an old paradigm. Diabetes. 1999; 45:1-9.

35

35. Latha M, Pari L, Sitasawad S, Bhonde R. Insulin-secretagogue activity and cytoprotective role of the traditional antidiabetic plant Scopariadulcis (Sweet Broom weed). Life Sciences. 2004;75:2003-2014.

상단으로 이동

Journal of Korean Medicine