- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Effects of Kyejiinsam-tang in MIA-Induced Osteoarthritis Rats
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2013, v.34 no.3, pp.69-85
&
(2013). Effects of Kyejiinsam-tang in MIA-Induced Osteoarthritis Rats. Journal of Korean Medicine, 34(3), 69-85.
- Downloaded
- Viewed
Abstract
Objectives: This study investigated the anti-osteoarthritic effects of Kyejiinsam-tang (hereinafter referred to KIT) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods:<in vitro>Anti-oxidative effects of KIT were measured by scavenging activities of DPPH, reactive oxygen species (ROS) and nitric oxide (NO). Scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cells were also measured for inhibitory effects against the production of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, interleukin-6). <in vivo>Osteoarthritis was induced in rats by injecting MIA in the knee joint. Rats were divided into a total of 4 groups (n=6). The normal group were not treated at all without inducing osteoarthritis whereas the control group were induced for osteoarthritis by MIA and oral medicated physiological saline per day. The positive comparison group was injected with MIA and after 7 days, 2 ㎎/㎏ of Indomethacin. The experimental group was injected with MIA and after 7 days was medicated with 34 ㎎/㎏ of KIT. Indomethacin and KIT were orally-medicated for each substance a total of 4 weeks, once per day. Weight-bearing on hind legs was measured every week after MIA injection. At the end of the experiment (5 weeks after MIA injection), micro CT (computed tomography)-arthrography and histopathological examinations on the articular structures of knee joint were performed. The effect on inflammatory cytokines and immunological cells in synovial fluid was measured. Volume of cartilage was measured by micro CT-arthrography. Injury to synovial tissue was measured by H & E (hematoxylin and eosin), Safranin-O immunofluorescence. Results: 1. Cytotoxicity against hFCs was insignificant. 2. KIT showed the potent full term for DPPH. <in vitro>1. NO was significantly reduced by KIT (at 100, 200 ㎍/㎖) and ROS was also reduced, but not significantly, by KIT (at 200 ㎍/㎖). 2. IL-6 and IL-1β were significantly reduced by KIT (at 100, 200 ㎍/㎖) and TNF-α was also reduced, but not significantly, by KIT (at 200 ㎍/㎖). <in vivo>1. In hind legs weight-bearing measurement, level of weight increased. 2. Functions of liver and kidney were not affected. 3. IL-1β was significantly reduced and TNF-α, IL-6 were also reduced but not significantly. 4. PGE2 (prostaglandin E2), LTB4 (leukotriene B4) were significantly reduced in the KIT group. 5. MMP-9 (matrix metalloproteinase-9), TIMP-1 (tissue inhibitor of metalloproteinases-1) and Osteocalcin were significantly reduced in the KIT group. 6. Destruction of cartilage on micro CT arthrography was reduced but had no significant differences. 7. Histopathologically, injury to synovial membrane of the KIT group was decreased and proteoglycan content of KIT group was increased. Conclusions: According to this study, Kyejiinsam-tang has inhibiting effect on the progression of arthritis in MIA-induced osteoarthritis rat. Kyejiinsam-tang has anti-oxidants and anti-inflammation effects, and is related to inhibiting the activity of inflammatory cytokine and injury of volume in cartilage.
- keywords
- Kyejiinsam-tang, osteoarthritis, anti-inflammation, anti-oxidation, MIA-induced.
Reference
1
1. The Korean Academy of Oriental RehabilitationMedicine. Oriental Rehabilitation Medicine 3rd..Seoul:KOONJA publishing inc. 2011:84.
2
2. Eugene Braunwald, A Fauci, D Kasper, SHauser, D. Longo. The Korean Association ofInternal Medicine. Harrison's principles of internalmedicine 16th ed. Seoul:MIP Publisher.2006:2083-4,2222-7.
3
3. Yoh SB, Sul JU, Shin MS. Research trends onthe treatment of knee Osteoarthritis in Koreanmedicine. The Korean Journal of Acupuncture.2011;28(1): 139-55.
4
4. Park BH. The commentary sanghanron clinicalapplications. Kangwon:Uibang publisher. 2004:317-8.
5
5. Kim SJ, Park SM, Kang H, Shim BS, Kim SH,Choi SH, et al. Anti-inflammatory effect ofCinnamomi ramulus on collagen induced arthritis;a model for rheumatoid arthritis in DBA/1Jmice and cytokine production in raw 264.7 cells.The Korean Journal of Oriental Physiology &Pathology. 2008;22(3):542-7.
6
6. Park EK, Shin YW, Lee HU, Kim SS, Lee YC,Lee BY, et al. Inhibitory effect of ginsenosideRb1 and compound K on NO and prostaglandinE2 biosynthesis of RAW 264.7 cells induced bylipopolysaccharide. Biol Pharm Bull. 2005;28:652-6.
7
7. Jung TY. Inhibitory effect of Korean ginseng extracton type II collagen-induced arthritis. Seoul:Graduate school, Yonsei Univ.; 2009.
8
8. Kim SH, Park YG. Effects of AtractylodisRhizoma Alba extract on collagen-induced arthritisin mice. The Korean Journal of herbology.2012;27(3):1-6.
9
9. Park JP, Son JH, Kim YM, Lee EY, Lim KH,Kim EH. Suppression of inflammatory macrophageresponse by Glycyrrhiza Uralensis herbalacupuncture extract. The Korean Journal ofAcupuncture. 2011; 28(4):49-58.
10
10. Ryu HS, Kim HS. Effect of Zingiber officinaleroscoe extracts on mice immune cell activation.The Korean Journal of Nutrition Korean. 2004;37(1):23-30.
11
11. Lee SY. The new gobangchancha. Seoul:Koonjapublisher. 2012:72.
12
12. TM. Piscaer, JH Waarsing, N Kops, PPavljasevic, JAN Verhaar, GJVM. van Osch, etal. In vivo imaging of cartilage degeneration usingμCT-arthrography. Osteoarthritis andCartilage. 2008;16(9):1011-7.
13
13. Murphy JM, Dixon K, Beck S, Fabian D,Feldman A, Barry f. Reduced chondrogenic andadipogenic activity of mesenchymal stem cellsfrom patients with advanced osteoarthritis.Arthritis and Rheum. 2002;46(3):704-13.
14
14. Yun JH. Osteoarthritis update. The KoreanJournal of Medicine. 2012;82(2):170-4.
15
15. Goldring MB, Otero M, Plumb DA, DraqomirC, Favero M, El Hachem K, et al. Roles of inflammatoryand anabolic cytokines in cartilagemetabolism:signals and multiple effectors convergeupon MMP-13 regulation in osteoarthritis.Eur Cell Mater 2011;21:202-220.
16
16. Jhun HJ, Ahn K, Lee SC. Estimation of the prevalenceof osteoarthritis in Korean adults basedon the data from the fourth Korea national healthand nutrition examination survey. Anesth PainMed 2010;5:201-206.
17
17. Walter R.Frontera. Muscular skeletal disease.Seoul:Koonja publisher. 2003:401.
18
18. Liu CC. Anti-Oxidants and Anti-inflammationEffects of Geongangbuja-tang. Daejoen:Graduate school, Daejoen Univ; 2011.
19
19. Choi WJ. The antioxidative and anti-inflammatoryeffects of lophatheri extracts forherbal-acupuncture via reducing iNOS synthesisinduced by LPS in RAW 264.7 Cell. Iksan:Graduated school, Wonkwong Univ.; 2010.
20
20. Park SD. Effect of Cervi Pantotrichum Cornuherbal acupuncture on protease activities antioxidantin rheumatoid arthritis rats. The Journalof Korean Acupuncture and MoxibustionSociety. 2002;19(2):51-64.
21
21. Sung YS, Choi HJ, Oh JM, Ji JG, Bak JW, KimDH. The effect of KKBT in papain-induced osteoarthritismice models. The Journal of DaejeonOriental Medicine. 2012;21(1):33-52.
22
22. Textbook Compilation Committee, College ofOriental Medicine, the National sanghanron.Determined sanghanron. Seoul:Ilzungsa. 2003:342-3.
23
23. Gil Ik-dong-dong . Yakjing. Seoul:CheonghongPublisher. 2006:105,116, 129,203,216.
24
24. Yoo YC. Role of nitric oxide in the nickel andcobalt induced cytotoxicity in RAW 264.7 cell.The Korean Journal of Occupational andEnvironmental Medicine. 2001;13(3):274-7.
25
25. Matsukawa A, Ohkawara S, Maeda T, Takagi K,Yoshinaga M. Production of IL-1 and IL-1 receptorantagonist and the pathological significancein lipolysis-accharideinduced arthritis.Clin Exp Immunol. 1993;93(2):206-11.
26
26. Cho HJ, Jang JB, Jung JU, Sung SC, Kim TK.Prevalence of radiographic knee osteoarthritis inelderly koreans. Knee surgery & related research.2009;21:223-31.
27
27. Bove S.E., Calcaterra S.L., Brooker R.M., HuberC.M., Guzman R.E., Juneau P.L., et al. Weightbearing as a measure of disease progression andefficacy of anti-inflammatory compounds in amodel of monosodium iodoacetate-inducedosteoarthritis. Osteoarthritis Cartilage. 2003;11(11):821-30.
28
28. Kim WY, Choi JB. Effects of Bee venom andCervi Cornu Parvum pharmacoacupuncture inmonosodium iodoacerare(MIA)-induced osteoarthritisrat. Journal of Oriental RehabilitationMedicine. 2010;20(1):61-77.
29
29. Janusz MJ, Hookfin EB, Heitmeyer SA,Woessner JF, Freemont AJ, Hoyland JA, et al.Moderation of iodoacetate-induced experimentalosteoarthritis rats by matrix metalloproteinaseinhibitors. Osteoarthritis Cartilage. 2001;9:751-60.
30
30. Guzman RE, Evans MG, Bove S, Morenko B,Kilgore K. Mono-iodoacetate-induced histologicchanges in subchondralbone and articular cartilageof rat femorotibial joints: an animal modelof osteoarthritis. Toxicol Pathol. 2003;31(6):619-24.
31
31. Kim NJ, Park JY, Han HC, Chang SH. Analgesicand anti-inflammatory effects of Ibuprofen,Indomethacin and NS-398 on an acute model ofarthritis in rats. Korean Journal ofAnesthesiology. 2001;40:802-13.
32
32. Ahn SI, Heuing BJ, Son JY. Antioxidative activityand nitrite scavenging abilities of some phenoliccompounds. Korean J. Food Cookery Sci.2007;23:19-24.
33
33. Kim SJ. DPPH radical scavenging and ACE inhibitoryeffects of the aerial parts of Fagopyrumesculentum, and isolation of flavonoids. MSthesis. Sunchon: Sunchon National Univ.; 2004.
34
34. Moncada S, Palmer RMJ, Higgs EA. NitricOxide: physiology, pathophysiology, andpharmacology. Pharmacol Rev. 1991;43:109-42
35
35. Wink DA, Mitchell JB. Chemical biology of nitricoxide: Insights into regulatory, cytotoxic, andcytoprotective mechanisms of nitric oxide. FreeRadic Biol Med. 1998;25(4-5):434-56.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Identification of Morphological Appearance of Fine Seed Herbs Using Stereoscope (ReportⅠ) - Celosiae Semen, Celoisae Cristatae Semen, Cuscutae Semen, Perillae Semen
- Availability of Diagnosis of Yin-deficiency in Elderly People with Xerostomia and Factors Influencing Subjective Oral Dryness: A Prospective Cross-sectional Study
- Anti-inflammatory Effect of Zeae Stigma Herbal-acupuncture at KI10 on LPS-induced Nephritis in Rats
- Student Satisfaction Study of Clinical Skills Training in Korean Medical Education
- Effects of Inhalable Microparticles of Socheongryong-tang on Chronic Obstructive Pulmonary Disease in a Mouse Model
- Effects of Kyejiinsam-tang in MIA-Induced Osteoarthritis Rats
- Observation of Inflammatory Marker Levels in Sprague-Dawley Rats with Youngyopaedoc-san-related Anti-atherogenic Effect
- Developing Questionnaire of BiJeung(痺證) which is similar to Arthralgia and Paresthesia Syndrome
- The Effect of Mild Cognitive Impairment Management Program on Traditional Korean Medicine in a Public Health Center
- Analysis of Patients Visiting an Oriental Hospital with Idiopathic Chronic Fatigue or Chronic Fatigue Syndrome
- Effects of Bogijetong-Tang on Diabetic-peripheral Neuropathy Induced by Streptozotocin in the Mouse
- Relationship Between Nutrient Intakes and Blood Biochemical Parameters of Korean Female Subjects Classified by Eight Constitution Medicine
- The Effect of Oriental Medicine Music Therapy on the Improvement of Elementary School Children's Concentration: Retrospective Analysis of Case Series
- Four Treatment Cases of Psoriasis of Soyangins Demonstrated by Yang Poison Exanthema
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.