- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Log In/Sign Up
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Evaluation of the in vivo skin moisturizing effects and underlying mechanisms of pomegranate concentrate solution and dried pomegranate concentrate powder
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2016, v.37 no.2, pp.12-22
(Health-Love Co., Ltd.)
(Research Institute, Health-Love Co., Ltd., Anyang)
(Research Institute, Health-Love Co., Ltd., Anyang)
(Research Institute, Health-Love Co., Ltd., Anyang)
(Research Institute, Health-Love Co., Ltd., Anyang)
Seung-Hee,
K.
, Hae-Yeon,
Y.
, Hye-Rim,
P.
, Mi-Sun,
S.
, &
(2016). Evaluation of the in vivo skin moisturizing effects and underlying mechanisms of pomegranate concentrate solution and dried pomegranate concentrate powder. Journal of Korean Medicine, 37(2), 12-22.
- Downloaded
- Viewed
Abstract
Objectives: The aim of this study was to confirm the dose-dependent skin moisturizing effects of dried pomegranate concentrate powder (PCP) and pomegranate concentrate solution (PCS) in ICR mice. Materials and methods: To observe the in vivo skin moisturizing effects and possible underlying mechanisms of PCP and PCS, oral PCP (100, 200, and 400 mg/kg) and PCS (1, 2, and 4 mL/kg) were administered to normal ICR mice. Changes in body weight, skin water content, and skin type I collagen and hyaluronan contents were measured. Additionally, the mRNA expression levels of hyaluronan synthase (Has) 1, 2, and 3, and collagen type I alpha (COL1A) 1 and 2 were determined in the dorsal skin of mice by real-time reverse transcription polymerase chain reaction (RT-PCR). Results: Significant and dose-dependent increases in dorsal skin water content and type I collagen and hyaluronan contents were seen in PCP and PCS-treated mice. Moreover, the mRNA levels of Has 1, 2, and 3, involved in hyaluronan synthesis, and of COL1A1 and COL1A2, involved in collagen synthesis, were significantly and dose-dependently upregulated in PCS- and PCP-treated mice. Conclusions: In this study, PCP and PCS led to favorable skin moisturizing effects as indicated by increased skin water content and the upregulation of hyaluronan and collagen synthesis enzymes in mice treated with PCS (4 mL/kg) and PCP (200 mg/kg).
- keywords
- Pomegranate concentrate solution, dried pomegranate concentrate powder, skin moisturizing effects, mouse, collagen, hyaluronan
Reference
1
1. Gilchrest BA. Skin aging and photoaging. Dermatol Nurs. 1990;2(2):79-82.
2
2. Wiedow O, Schröder JM, Gregory H, Young JA, Christophers E. Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. J Biol Chem. 1990;265(25):14791-14795.
3
3. Maity N, Nema NK, Abedy MK, Sarkar BK, Mukherjee PK. Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity. J Ethnopharmacol. 2011;137(3):1300-1305.
4
4. Chan YY, Kim KH, Cheah SH. Inhibitory effects of Sargassum polycystum on tyrosinase activity and melanin formation in B16F10murine melanoma cells. J Ethnopharmacol. 2011;137(3):1183-1188.
5
5. Imokawa G. Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibrobrast-derived elastase. Arch Dermatol Res. 2008;300 Suppl 1:S7-20.
6
6. Kim EJ, Kim MK, Jin XJ, Oh JH, Kim JE, Chung JH. Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin. J Korean Med Sci. 2010;25(6):980-983.
7
7. Dziezak JD. Preservatives: antioxidants. The ultimate answer to oxidation. Food Technology. 1986;40(9):94-102.
8
8. Cho EA, Cho EH, Choi SJ, Park KH, Kim SY, Jeong YJ, et al. Screening of anti-wrinkle resource from herbal medicinal extracts and stability test of its cosmetic products. Korean Journal of Medicinal Crop Science. 2011;19(2):126-135.
9
9. Kim KH, Park SJ, Lee JE, Lee YJ, Song CH, Choi SH, et al. Anti-skin-aging benefits of exopolymers from Aureobasidium pullulans SM2001. J Cosmet Sci. 2014;65(5):285-298.
10
10. Kim YH, Chung CB, Kim JG, Ko KI, Park SH, Kim JH, et al. Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem. 2008;72(2):303-311.
11
11. Losso JN, Munene CN, Bansode RR, Bawadi HA. Inhibition of matrix metalloproteinase-1activity by the soybean Bowman-Birk inhibitor. Biotechnol Lett. 2004;26(11):901-905.
12
12. Parvez S, Kang M, Chung HS, Cho C, Hong MC, Shin MK, et al. Survey and mechanism of skin depigmenting and lightening agents. Phytother Res. 2006;20(11):921-934.
13
13. Parvez S, Kang M, Chung HS, Bae H. Naturally occurring tyrosinase inhibitors: mechanism and applications in skin health, cosmetics and agriculture industries. Phytother Res. 2007;21(9):805-816.
14
14. Camargo FB, Jr., Gaspar LR, Maia Campos PM. Skin moisturizing effects of panthenol-based formulations. J Cosmet Sci. 2011;62(4):361-370.
15
15. Kanlayavattanakul M, Rodchuea C, Lourith N. Moisturizing effect of alcohol-based hand rub containing okra polysaccharide. Int J Cosmet Sci. 2012;34(3):280-283.
16
16. Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem. 2000;48(10):4581-4589.
17
17. Noda Y, Kaneyuki T, Mori A, Packer L. Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin. J Agric Food Chem. 2002;50(1):166-171.
18
18. Singh RP, Chidambara Murthy KN, Jayaprakasha GK. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem. 2002;50(1):81-86.
19
19. Kim ND, Mehta R, Yu W, Neeman I, Livney T, Amichay A, et al. Chemopreventive and adjuvant therapeutic potential of pomegranate (Punica granatum) for human breast cancer. Breast Cancer Res Treat. 2002;71(3):203-217.
20
20. Kang SJ, Choi BR, Kim SH, Yi HY, Park HR, Park SJ, et al. Inhibitory effects of pomegranate concentrated solution on the activities of hyaluronidase, tyrosinase, and metalloproteinase. J Cosmet Sci. 2015;66(3):145-159.
21
21. Kang SJ, Choi BR, Kim SH, Yi HY, Park HR, Song CH, et al. Effect of pomegranate concentration solution on photoaging. J Soc Prev Korean Med. 2015;19(1):109-116.
22
22. Oishi Y, Kato H, Noguchi T. Dietary protein as a potent regulator of the hyaluronan synthase gene in rat skin. Biosci Biotechnol Biochem. 2003;67(4):736-742.
23
23. Yamane T, Nakagami G, Yoshino S, Muramatsu A, Matsui S, Oishi Y, et al. Hydrocellular foam dressing promotes wound healing along with increases in hyaluronan synthase 3 and PPARa gene expression in epidermis. PLoS One. 2013;8(8):e73988.
24
24. Arriazu E, Ruiz de Galarreta M, López-Zabalza MJ, Leung TM, Nieto N, Iraburu MJ. GCN2kinase is a key regulator of fibrogenesis and acute and chronic liver injury induced by carbon tetrachloride in mice. Lab Invest. 2013;93(3):303-310.
25
25. Kwak YJ, Kim NM, Lee DH, Lee JS. Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Korean J Medicinal Crop Sci. 2005;13:213-216.
26
26. Kang SJ, Lee JE, Lee EK, Jung DH, Song CH, Park SJ, et al. Fermentation with Aquilariae Lignum enhances the anti-diabetic activity of green tea in type II diabetic db/db mouse. Nutrients. 2014;6(9):3536-3571.
27
27. Jacobi OK. About the mechanism of moisture regulation in the horny layer of the skin. Proc Scient Sect Toil Goods Assoc. 1959;31:22-26.
28
28. Liao PL, Li CH, Chang CY, Lu SR, Lin CH, Tse LS, et al. Anti-ageing effects of alpha -naphthoflavone on normal and UVB-irradiated human skin fibroblasts. Exp Dermatol. 2012;21(7):546-548.
29
29. Wallis GA, Sykes B, Byers PH, Mathew CG, Viljoen D, Beighton P. Osteogenesis imperfecta type III: mutations in the type I collagen structural genes, COL1A1 and COL1A2, are not necessarily responsible. J Med Genet. 1993;30(6):492-496.
30
30. Frenkel JS. The role of hyaluronan in wound healing. Int Wound J. 2014;11(2):159-163.
31
31. Weigel PH, Hascall VC, Tammi M. Hyaluronan synthases. J Biol Chem. 1997;272(22):13997-14000.
32
32. Kim SH, Nam GW, Lee HK, Moon SJ, Chang IS. The effects of Musk T on peroxisome proliferator-activated receptor [PPAR]-a activation, epidermal skin homeostasis and dermal hyaluronic acid synthesis. Arch Dermatol Res. 2006;298(6):273-282.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Effect of Jaeumkanghwatang (JEKHT), a Polyherbal Formula on the Pharmacokinetics Profiles of Tamoxifen in Male SD Rats (1) - Single Oral Combination Treatment of Tamoxifen 50mg/kg with JEKHT 100 mg/kg within 5 min -
- Evaluation of the in vivo skin moisturizing effects and underlying mechanisms of pomegranate concentrate solution and dried pomegranate concentrate powder
- Cardioprotective Effects of Salvia Miltiorrhiza Radix on the Pressure Overloaded Heart Failure Model by Transverse Aortic Constriction-induced Mice
- The Effects of Breath-Counting Meditation and Deep Breathing on Heart Rate Variability
- The Clinical Research of the Effectiveness of Muscle Energy Technique (MET) on Peripheral Facial Paralysis
- The Effect of Periocular Acupressure with a Medical Massager for Myopia Children
- The Effects of Lycii Radicis Cortex on Inflammatory Response through an Oxidative Stress and AGEs-mediated Pathway in STZ-induced Diabetic Rats
- Rh2-enriched Korean Ginseng Ameliorates Chronic Fatigue in a Forced Exercise mouse model
- Evaluation of the effects of a low dose of Asiasari radix on stem cell morphology and proliferation
- Non-operative Korean Medicine Treatment for Four Patients with Failed Back Surgery Syndrome after Spinal Fusion Surgery : A Retrospective Case Series
- Improvement of Chronic Bleeding in the Patient with Unresectable Advanced Gastric Cancer Using the Decoction of Notoginseng Radix - a Case Report
- Treatment of Psoriasis with Exudation: Three Case Studies
- A Severe Hepatotoxicity by Antituberculosis Drug, and its Recovery in Oriental Hospital
- The clinical effect of Embedding Therapy for Ramsay Hunt syndrome
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.