- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Log In/Sign Up
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Evaluation of the effects of a low dose of Asiasari radix on stem cell morphology and proliferation
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2016, v.37 no.2, pp.85-92
&
(2016). Evaluation of the effects of a low dose of Asiasari radix on stem cell morphology and proliferation. Journal of Korean Medicine, 37(2), 85-92.
- Downloaded
- Viewed
Abstract
Objectives: Asiasari radix (A. radix) is a traditional herb medicine that has been used as an analgesic, antitussive, or anti-allergic remedy. This study was performed to evaluate the effects of low concentration of the Asiasarum heterotropoides extract on the morphology and proliferation of the human mesenchymal stem cells derived from periodontal tissue. Methods: Stem cells derived from gingiva were grown in the presence of A. radix at final concentrations that ranged from 0.001 to 0.01 μg/mL. The morphology of the cells was viewed under an inverted microscope and the analysis of cell proliferation was performed by using Cell Counting Kit-8 (CCK-8) on days 1 and 3. Results: The control group showed fibroblast morphology. The shapes of the cells in 0.001 and 0.01 μg/mL of A. radix were similar to that of the untreated control group. The cultures growing in the presence of A. radix at day 1 showed an increase in the CCK-8 value. The relative values of CCK-8 assays of 0.001 and 0.01 μg/mL of A. radix are 130.6 % ± 1.8 % and 129.3 % ± 1.5 %, respectively, when the CCK-8 result of the untreated control group at day 1 is considered 100% (P = 0.051). Conclusions: Within the limits of this study, low concentrations of A. radix seemed to increase the proliferation of the stem cells that were derived from the gingiva and did not have adverse effects on the morphology of the cells.
- keywords
- Asiasari Radix, low dose, stem cells, cell morphology, cell proliferation
Reference
1
1. Zhou RH. Resource science of Chinese medicinal materials. Beijing: China Medical & Pharmaceutical Sciences Press. 1993:202-211.
2
2. Jang JY, Lee JH, Shin HK, Choi YH, Lee JD, Choi BT. Partially purified Asiasari radix inhibits melanogenesis through extracellular signal-regulated kinase signaling in B16F10cells. Int J Mol Med. 2010;25:287-292.
3
3. Jeong SH, Lee JE, Jin SH, Ko Y, Park JB. Effects of Asiasari radix on the morphology and viability of mesenchymal stem cells derived from the gingiva. Mol Med Rep. 2014;10(6):3315-3319.
4
4. Kim HM, Moon YS. Asiasari radix inhibits immunoglobulin E production on experimental models in vitro and in vivo. Immunopharmacol Immunotoxicol. 1999;21:469-481.
5
5. Rho SS, Park SJ, Hwang SL, Lee MH, Kim CD, Lee IH, et al. The hair growth promoting effect of Asiasari radix extract and its molecular regulation. J Dermatol Sci. 2005;38:89-97.
6
6. Jeon HC, Rho EJ, Kim HR, Yun YG. A Study on application of radix Asari main blended prescription from Dongeubogam. Korean J Oreint med Prescr. 2004;12(2):57-76.
7
7. Takara K, Horibe S, Obata Y, Yoshikawa E, Ohnishi N, Yokoyama T. Effects of 19 herbal extracts on the sensitivity to paclitaxel or 5-fluorouracil in HeLa cells. Biol Pharm Bull. 2005;28:138-142.
8
8. Oh SM, Kim J, Lee J, Yi JM, Oh DS, Bang OS, et al. Anticancer potential of an ethanol extract of Asiasari radix against HCT-116human colon cancer cells in vitro. Oncol Lett. 2013;5:305-310.
9
9. Jeong SH, Kim BB, Lee JE, Ko Y, Park JB. Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells. Mol Med Rep. 2015;12:1556-1560.
10
10. Jeong SH, Lee JE, Kim BB, Ko Y, Park JB. Evaluation of the effects of Cimicifugae Rhizoma on the morphology and viability of mesenchymal stem cells. Exp Ther Med. 2015;10:629-634.
11
11. Park JB. Restoration of the severely decayed tooth using crown lengthening with simultaneous tooth-preparation. European journal of dentistry. 2010;4:197-201.
12
12. Wang Y, Wang WL, Xie WL, Li LZ, Sun J, Sun WJ, et al. Puerarin stimulates proliferation and differentiation and protects against cell death in human osteoblastic MG-63 cells via ER-dependent MEK/ERK and PI3K/Akt activation. Phytomedicine. 2013;20: 787-796.
13
13. Fournier BP, Ferre FC, Couty L, Lataillade JJ, Gourven M, Naveau A, et al. Multipotent progenitor cells in gingival connective tissue. Tissue Eng Part A. 2010;16:2891-2899.
14
14. Yang H, Gao LN, An Y, Hu CH, Jin F, Zhou J, et al. Comparison of mesenchymal stem cells derived from gingival tissue and periodontal ligament in different incubation conditions. Biomaterials. 2013;34: 7033-7047.
15
15. Su WR, Zhang QZ, Shi SH, Nguyen AL, Le AD. Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanisms. Stem Cells. 2011;29:1849-1860.
16
16. Zhang Q, Nguyen AL, Shi S, Hill C, Wilder -Smith P, Krasieva TB, et al. Three-dimensional spheroid culture of human gingiva -derived mesenchymal stem cells enhances mitigation of chemotherapy-induced oral mucositis. Stem Cells Dev. 2012;21:937-947.
17
17. Fournier BP, Larjava H, Hakkinen L. Gingiva as a source of stem cells with therapeutic potential. Stem Cells Dev. 2013;22:3157-3177.
18
18. Tomar GB, Srivastava RK, Gupta N, Barhanpurkar AP, Pote ST, Jhaveri HM, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Commun. 2010; 393:377-383.
19
19. Chen M, Su W, Lin X, Guo Z, Wang J, Zhang Q, et al. Adoptive transfer of human gingiva-derived mesenchymal stem cells ameliorates collagen-induced arthritis via suppression of Th1 and Th17 cells and enhancement of regulatory T cell differentiation. Arthritis Rheum. 2013;65:1181-1193.
20
20. Zhang Q, Shi S, Liu Y, Uyanne J, Shi Y, Le AD. Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis. J Immunol. 2009;183:7787-7798.
21
21. Zhang QZ, Su WR, Shi SH, Wilder-Smith P, Xiang AP, Wong A, et al. Human gingiva-derived mesenchymal stem cells elicit polarization of m2 macrophages and enhance cutaneous wound healing. Stem Cells. 2010;28:1856-1868.
22
22. Wang F, Yu M, Yan X, Wen Y, Zeng Q, Yue W, et al. Gingiva-derived mesenchymal stem cell-mediated therapeutic approach for bone tissue regeneration. Stem Cells Dev. 2011;20:2093-2102.
23
23. Lee SI, Yeo SI, Kim BB, Ko Y, Park JB. Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests. Biomed Rep. 2016;4:97-101.
24
24. Santamaria S, Sanchez N, Sanz M, Garcia-Sanz JA. Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies. Clin Oral Investig. 2016.
25
25. Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol. 2001;Appendix 3:Appendix 3B.
26
26. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
27
27. Meleti Z, Shapiro IM, Adams CS. Inorganic phosphate induces apoptosis of osteoblast-like cells in culture. Bone. 2000;27:359-366.
28
28. Vande Vannet B, Mohebbian N, Wehrbein H. Toxicity of used orthodontic archwires assessed by three-dimensional cell culture. Eur J Orthod. 2006;28:426-432.
29
29. Sutherland MW, Learmonth BA. The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase. Free Radic Res. 1997;27:283-289.
30
30. Shi MF, Jiao J, Lu WG, Ye F, Ma D, Dong QG, et al. Identification of cancer stem cell-like cells from human epithelial ovarian carcinoma cell line. Cell Mol Life Sci. 2010;67:3915-3925.
31
31. Zou C, Shen Z. An optimized in vitro assay for screening compounds that stimulate liver cell glucose utilization with low cytotoxicity. J Pharmacol Toxicol Methods. 2007;56:58-62.
32
32. Wagner U, Burkhardt E, Failing K. Evaluation of canine lymphocyte proliferation: comparison of three different colorimetric methods with the 3H-thymidine incorporation assay. Vet Immunol Immunopathol. 1999;70:151-159.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Effect of Jaeumkanghwatang (JEKHT), a Polyherbal Formula on the Pharmacokinetics Profiles of Tamoxifen in Male SD Rats (1) - Single Oral Combination Treatment of Tamoxifen 50mg/kg with JEKHT 100 mg/kg within 5 min -
- Evaluation of the in vivo skin moisturizing effects and underlying mechanisms of pomegranate concentrate solution and dried pomegranate concentrate powder
- Cardioprotective Effects of Salvia Miltiorrhiza Radix on the Pressure Overloaded Heart Failure Model by Transverse Aortic Constriction-induced Mice
- The Effects of Breath-Counting Meditation and Deep Breathing on Heart Rate Variability
- The Clinical Research of the Effectiveness of Muscle Energy Technique (MET) on Peripheral Facial Paralysis
- The Effect of Periocular Acupressure with a Medical Massager for Myopia Children
- The Effects of Lycii Radicis Cortex on Inflammatory Response through an Oxidative Stress and AGEs-mediated Pathway in STZ-induced Diabetic Rats
- Rh2-enriched Korean Ginseng Ameliorates Chronic Fatigue in a Forced Exercise mouse model
- Evaluation of the effects of a low dose of Asiasari radix on stem cell morphology and proliferation
- Non-operative Korean Medicine Treatment for Four Patients with Failed Back Surgery Syndrome after Spinal Fusion Surgery : A Retrospective Case Series
- Improvement of Chronic Bleeding in the Patient with Unresectable Advanced Gastric Cancer Using the Decoction of Notoginseng Radix - a Case Report
- Treatment of Psoriasis with Exudation: Three Case Studies
- A Severe Hepatotoxicity by Antituberculosis Drug, and its Recovery in Oriental Hospital
- The clinical effect of Embedding Therapy for Ramsay Hunt syndrome
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.