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Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • P-ISSN2234-7550
  • E-ISSN2234-5930
  • SCOPUS, KCI, ESCI

Xenografted tumorigenesis in the oral vestibule of nude mice by snail transfection: Histological and immunohistochemical study

Journal of the Korean Association of Oral and Maxillofacial Surgeons / Journal of the Korean Association of Oral and Maxillofacial Surgeons, (P)2234-7550; (E)2234-5930
2009, v.35 no.4, pp.199-204
김문기 (국민건강보험공단)
이은하 (연세대학교)
김진 (연세대학교)
육종인 (연세대학교)
차인호 (연세대학교)

Abstract

Purpose: The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfectedin vivo tumors with histopathological features. Materials and methods: We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell lineand investigated morphological and immunohistochemical features in Snail expressive tumors. Results: We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of theHaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones. The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin. Discussion: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasivegrowth, and aid in the investigation of tumor progression and the interaction with surrounding tissues.

keywords
E-cadherin, neoplastic cell transformation, Snail transcription factor, xenograft model

Journal of the Korean Association of Oral and Maxillofacial Surgeons