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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

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대식세포에서 산화질소 생성에 대한 당귀 에탄올 추출물의 억제 효과

Inhibitory Effect of Angelica gigias Nakai Extrcact on Nitric Oxide Production in RAW 264.7 Cells

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2007, v.28 no.2, pp.155-165
정미영 (경희대학교)
박히준 (경희대학교)
정지행 (정지행리듬케어㈜)
김진용 (경희대학교)
강전모 (경희대학교)
이나경 (경희대학교)
임사비나 (경희대학교)
정미영, 박히준, 정지행, 김진용, 강전모, 이나경, & 임사비나. (2007). 대식세포에서 산화질소 생성에 대한 당귀 에탄올 추출물의 억제 효과. 대한한의학회지, 28(2), 155-165.
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Abstract

Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and NF-κB activity from RAW 264.7 cells, since NO and nuclear factor-κB (NF-κB) have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of NF-κB on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 μg/ml. LPS-induced RAW 264.7 cells with AGE blocked inhibitory factor-κBα degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB activation in LPS-stimulated RAW 264.7 cells.

keywords
Angelica gigas Nakai, RAW 264.7 cells, inflammation, nitric oxide, nuclear factor κB, Angelica gigas Nakai, RAW 264.7 cells, inflammation, nitric oxide, nuclear factor κB


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