- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Inhibitory Effect of Angelica gigias Nakai Extrcact on Nitric Oxide Production in RAW 264.7 Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2007, v.28 no.2, pp.155-165
&
(2007). Inhibitory Effect of Angelica gigias Nakai Extrcact on Nitric Oxide Production in RAW 264.7 Cells. Journal of Korean Medicine, 28(2), 155-165.
- Downloaded
- Viewed
Abstract
Objective : The Angelica gigas Nakai ethanol extract (AGE) was investigated to compare nitric oxide (NO) production and NF-κB activity from RAW 264.7 cells, since NO and nuclear factor-κB (NF-κB) have been shown to be factors implicated in inflammatory disease. Method : AGE was prepared by extracting medicinal herb with 70% (v/v) ethanol solution. We investigated production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) gene expression by ARE in LPS-stimulated RAW 264.7 macrophage cells. We also investigated inhibition of LPS-induced activation of NF-κB on western blot. Result : LPS-induced RAW 264.7 cells increased NO production and iNOS expression. Upon treatment with AGE, nitrite production was significantly inhibited in a concentration-dependent manner compared to the untreated control. AGE inhibited this LPS-induced iNOS mRNA and protein in a dose-dependent manner. AGE markedly inhibited the expression of iNOS mRNA and protein at a concentration of 100 μg/ml. LPS-induced RAW 264.7 cells with AGE blocked inhibitory factor-κBα degradation. Conclusion :This study shows that AGE seems to attenuate inflammation through inhibition of NO production and iNOS expression by blockade of NF-κB activation in LPS-stimulated RAW 264.7 cells.
- keywords
- Angelica gigas Nakai, RAW 264.7 cells, inflammation, nitric oxide, nuclear factor κB, Angelica gigas Nakai, RAW 264.7 cells, inflammation, nitric oxide, nuclear factor κB
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Neuroprotective Effect of Gagamchongmyung-tang on the Deficits of Learning and Memory by MCAO in the Rat
- Clinical Characteristics of Patients with Halitosis
- Study on Association of DSOM Items for Uterine Myoma in Oriental Medicine -Control Group:Outpatient and Clinical Trials Data-
- A Clinical Study about the Effect of Soshiho-tang on a Case of Parkinson's Disease with Pontine Infarction
- Neuroprotective Effect of Hyulbuchookau-tang on Global Cerebral Ischemia of the Rats
- Effect of Naenghyo-hwan on Secretion of Airway Mucin and Contractility of Tracheal Smooth Muscle
- Short Term Reaction to Acupuncture Treatment and Adverse Events Following Acupuncture in Korea a Cross-sectional Survey of Patient Reports
- A Clinical Study on the Relationship between Functional Dyspepsia(FD) and Biosignals from Heart Rate Variability(HRV) and Yangdorak Diagnosis
- An Analysis of Tendencies of Studies on Herbal Acupuncture -Focusing on domestic theses since 2001 about anti-inflammation, pain relief and anti-obesity effects, including safety-
- A study on Skin Resistance Variability (SRV) of Woman with Uterine Myoma
- The effect of Kuwonsimsin-hwan on Imunosuppression Induced by Methotrexate in Rat
- The Effects of Gagbyungyunsoo-tanghap Zeungsonbakchul-san(GYZB) Hot Water Extract & Ultra-fine Powder on the Alzheimer's Disease Model
- Inhibitory Effect of Angelica gigias Nakai Extrcact on Nitric Oxide Production in RAW 264.7 Cells
- Effects of Daegeum-eumja, Igwi-tang Sihosogan-san on Gastric Mucosal Lesions Induced by Alcohol, Indomethacin and Burn-stress in Mice
- Damsam-san Inhibits Atherosclerosis through Regulation of Foam Cell Formation and Vascular Smooth Muscle Proliferation
- Acute Toxicity Study on Sagunja-tang(Sijunzi-tang) in ICR Mice
- Combined Treatment of Oriental Herbal Medicine and Prescribed Drugs among Cancer Patients
- Study of Apoptotic Effect on Hydrogen Peroxide-induced Rat PC-12 Cells by Aster tataricus Water Extract
- The Effects of Jiyutang against Gastric and Ileac Mucosal Ulcer Induced by Indomethacin in Mouse
- Acute Stroke in the Elderly Male -Clinical Features,Stroke Subtypes, and Sasang Constitutions-
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.