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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

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냉효환이 호흡기 뮤신 분비와 기관지 평활근에 미치는 영향

Effect of Naenghyo-hwan on Secretion of Airway Mucin and Contractility of Tracheal Smooth Muscle

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2007, v.28 no.2, pp.54-65
윤종만 (대전대학교 한의과대학)
박양춘 (대전대학교)
이용구 (대전대학교 혜화병원)
윤종만, 박양춘, & 이용구. (2007). 냉효환이 호흡기 뮤신 분비와 기관지 평활근에 미치는 영향. 대한한의학회지, 28(2), 54-65.
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Abstract

Objectives : In the present study, the author investigated whether Naenghyo-hwan(NHH) significantly affect mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of NHH to assess the effect of the agent on 3H-mucin secretion. Total elutionprofiles of control spent media and treatment sample through Sepharose CL-4B column were analysed. Effect of NHH on contractility of isolated tracheal smooth muscle was investigated. Also, effect of the agent on MUC5AC gene expression in cultured NCI-H292cells was investigated. Possible cytotoxicities of the agent were assessed by measuring both lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : NHH significantly increased mucin secretion from cultured HTSE cells, with significant cytotoxicity. NHH chiefly affected the 'mucin' secretion. NHH inhibited ACh-induced contraction of isolated tracheal smooth muscle. NHH disturbed both the extraction of total RNA from NCI-H292 cells and polymerase chain reaction, nonspecifically. Therefore, in this experiment, theeffect of NHH on the expression levels of MUC 5AC gene in cultured NCI-H292 cells could not be elucidated. Conclusions : The author suggests that the effect of NHH with their components should be further investigated and it is valuable to find, from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.

keywords
airway, mucin, Naenghyo-hwan (NHH), MUC5AC mRNA, tracheal smooth muscle, airway, mucin, Naenghyo-hwan (NHH), MUC5AC mRNA, tracheal smooth muscle


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