- P-ISSN 1010-0695
- E-ISSN 2288-3339
Background & Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycanand collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of Cinnamomum cassia in inhibiting the release of glycosaminoglycan (GAG), the degradation of collagen, and MMP activity in rabbit and human articular cartilage explants. Methods: The cartilage-protective effects of Cinnamomum cassia were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMP activity, measurement of lactate dehydrogenase activity and histological analysis in rabbit cartilage explants culture. Results: Interleukin-1a (IL-1a) rapidly induced GAG, but collagen was much less readily released from cartilage explants. Cinnamomum cassia significantly inhibited GAG and collagen release in a concentration-dependent manner. Cinnamomum cassia dose-dependently inhibited MMP-1, MMP-3 and MMP-13 activities from IL-1a-treated cartilage explants culture when tested at concentrations ranging from 0.02 to 1 mg/ml. Conclusion : These results indicate that Cinnamomum cassia inhibits the degradation of proteoglycan and collagen through the down regulation of MMP-1, MMP-3 and MMP-13 activities of IL-1a-stimulated rabbit and human articular cartilage explants.
(1995) The struc- ture of aggrecan and its turnover in cartilage,
S. There are two major types of skeletal keratan sulphates. Biochemical Journal. 1990,
H. Localization of matrix metalloproteinase 3,
M.W. Detection of stromelysin and collagenase in synovial fluid from patients with rheumatoid arthritis and posttraumatic knee injury. Arthritis and Rhe- umatism. 1992,
T.E. The prevention of collagen breakdown in bovine nasal cartilage by TIMP,
(2007) Effects of glucosamine and chondroitin sulfate on bovine cartilage explants under long-term culture conditions,
(1998) Involvement of MMP-1 and MMP-3 in collagen degradation induced by IL-1 in rabbit cartilage explant culture,
(2003) Serum MMP-3 and MMP-1 progression of joint damage in early rheumatoid arthritis,
(1997) Collagenase- 3 (matrix metalloproteinase-13) is prefer- entially localizedin the deep layer of human arthritic cartilage in situ In vitro mimicking effect by transforming growth factor,
(1996) Biochemical chara- cterizationof human collagenase-3,
(2005) Inhibitory constituents against cyclooxygenases from Aralia cordata Thunb,
M. Analgesic principles from Aralia cordata Thunb. Chemical Pharmaceutical Bulletin. 1991,
(1998) Intra-articular injection of collagenase ind- uces experimental osteoarthritis in mature rabbits,
(1999) Inhibition of interleukin-1-induced proteo- glycan degradation and nitric oxide produ- ction in bovine articular cartilage/chondrocyte cultures by the natural product, hymeni- aldisine,
S. Studies on interleukin-1b induced glycosa- minoglycan release from rat femoral head cartilage in-vitro. Journal of Pharmacy and Pharmacology. 1989,
(195-206.) D.A. Reduction of the sevierity of canine osteoar- thritis by potential chondroprotective agents. Agents Actions. 1993,
(1995) Cyclooxygenase inhibitors augument the production of pro-matrix metalloproteinase 9 (progelatinase B) in rabbit articular chondr- ocytes,
(2004) Increased stromyelin-1 (MMP-3) prot- eoglycan degradation (3B3- and 7D4) and collagen demage in cyclically load injuried articular cartilage,
(2003) Glucosamin sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chon- drocytes,
(1998) Stromyelin 1, neutrophil collagenase, and collagenase 3 do not play major roles in a model of chondrocyte mediated cartilage breakdown,
(2007) From type 2 diabetes to antioxidant activity: a systematic review of the safety andefficacy of common and cassia cinnamon bark,
(2000) Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout,
(2000) Retinoic acid combines with interleukin-1 to promote the degradation of collagen from bovine nasal cartilage: Matrix Metalloproteinase-1 and 13 are involved in cartilage breakdown,
(2002) Effects of SKI306X, a new herbal agent, on proteoglycan degradation in cartilage explant culture and collagenase-induced rabb- itosteoarthritis model,
(1998) Stromyelin 1, neutrophil collagenase, and collagenase 3 do not play major roles in a model of chondrocyte mediated cartilage breakdown,
(2004) Increased stromyelin-1 (MMP-3), prote- oglycan degradation (3B3- and 7D4) and collagen demage in cyclically load injuried articular cartilage,