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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects of Woo-Gui-Um on Aβ Toxicity and Memory Dysfunction in Mice

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.3, pp.1-14










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Abstract

Objectives:Alzheimer’s disease (AD) is characterized by neuronal loss and extracellular senile plaque. Moreover, the cellular actions of β-amyloid (Aβ) play a causative role in the pathogenesis of AD. This study was designed to determine whether Woo-Gui-Um, a commonly used Korean herbal medicine, has the ability to protect cortical and hippocampal neurons against Aβ25-35 neurotoxicity Methods:In the present study, the authors investigated the preventative effects of the water extract of Woo-Gui-Um in a mouse model of AD. Memory impairment was induced by intraventricularly (i.c.v.) injecting Aβ25-35 peptides into mice. Woo-Gui-Um extract was then administered orally (p.o.) for 14 days. In addition, Aβ25-35 toxicity on the hippocampus was assessed immunohistochemically, by staining for Tau, MAP2, TUNEL, and Bax, and by performing an in vitro study in PC12 cells. Results:Woo-Gui-Um extract had an effect to improve learning ability and memory score in the water maze task. Woo-Gui-Um extract had significant neuroprotective effects in vivo against oxidative damage and apoptotic cell death of hippocampal neurons caused by i.c.v. Aβ25-35. In addition, Woo-Gui-Um extract was found to have a protective effect on Aβ25-35-induced apoptosis, and to promote neurite outgrowth of nerve growth factor (NGF)-differentiated PC12 cells. Conclusions:These results suggest that Woo-Gui-Um extract reduces memory impairment and Alzheimer’s dementia via an anti-apoptotic effect and by regulating Tau and MAP2 in the hippocampus.

keywords
Woo-Gui-Um, learning and memory, Aβ25-35, Tau, MAP2, Bax


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