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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

A Trend of Yin-tonifying Formulas Compared with Yang-tonifying Formulas on Anti-platelet and Anti-thrombotic Activity

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.6, pp.1-8
Won Kyung Jeon (Korea Institute of Oriental Medicine)
Bo Kyung Yoo (Korea Institute of Oriental Medicine)
Sang Young Ahn (Korea Institute of Oriental Medicine)
Ju Hyun Lee (Korea Institute of Oriental Medicine)
Sang Woo Ahn (Korea Institute of Oriental Medicine)

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Abstract

Objectives:Formulas for treatment of yin or yang deficiency conditions have been commonly used in traditional Korean medicine. The aim of this study is to examine the possible inhibitory effects of yin- or yang-tonifying formulas on in vivo anti-platelet activity and in vivo anti-thrombotic activity. Methods:We tested the effects of 26 types of yin- or yang-tonifying formulas on platelet aggregation induced by collagen in human whole blood using the impedance method of aggregometry and accessed a biomarker of platelet activation using thromboxane B2 immunoassay. We also tested the anti-thrombotic effects of effective candidates on experimental models of thrombosis in mice. Results:3 types of yin-tonifying formulas and 3 types of yin-yang-tonifying formulas were selected to be the most effective candidates (p<0.01). Also, through in vivo study, the antithrombotic activities of Igyeong-tang, Gamisipjeondaebo-tang, and Gamisoyo-san-treated groups, with recovery rate of 60, 50, and 45.45%, respectively, were observed to be higher than those of the control group (saline, 36.8%) in mouse acute thrombosis. Conclusion:These results show that yin-tonifying formulas are more effective in anti-platelet and anti-thrombotic activity than yang-tonifying formulas.

keywords
formulas, tonifying, yin, yang, platelet aggregation, antithrombotic


Reference

1

Song TW. Introduction to Traditional Korean Medicine. Daejeon: Korea Institute of Oriental Medicine. 2007.

2

Yamada H, Saiki I. Juzen-taiho-to (Shi-Quan-Da-Bu-Tang): Scientific Evaluation and Clinical Applications. CRC press. 2005: 1-6.

3

Hosoya E. Scientific evaluation of Kampo prescriptions using modern technology. In: Hosoya E, Yamamura T, editors. Recent advances in the pharmacology of Kampo (Japanese Herbal) Medicines. Amsterdam: Excerpta Medica. 1988:17-29.

4

Seki K, Chisaka M, Eriguchi M, Yanagie H, Hisa T, Osada I, et al. An attempt to integrate western and Chinese medicine: rationale for applying Chinese medicine as chronotherapy against cancer. Biomed Pharmacother. 2005; 59:S132-40.

5

Ko KM, Mak DH, Chiu PY, Poon MK, Poon MK. Pharmacological basis of ‘Yang-invigoration’ in Chinese medicine. Trends Pharmacol Sci. 2004; 25:3-6.

6

Yi YD, Chang IM. An Overview of Traditional Chinese Herbal Formulae and a Proposal of a New Code System for Expressing the Formula Titles. eCAM. 2004; 1:125-32.

7

Jeon WK, Yoo BK, Kim YE, Park SO, Park SM, Ko BS. Effect of extracts for herbal medicine on the inhibition of whole blood aggregation. J Korean Soc Appl Biol Chem. 2007; 50:352-7.

8

Jeon WK. Characterization of the bioactive compounds extracted from the bark of Rhus verniciflua Stokes and their anti-platelet and anti-obesity actions [PhD thesis]. Seoul, Korea: University of Konkuk.; 2006.

9

Jin YR, Cho MR, Ryu CK, Chung JH, Yuk DY, Hong JT, et al. Antiplatelet activity of J78 (2-Chloro-3-[2’-bromo, 4’-fluoro-phenyl]-amino-8-hydroxy-1,4-naphthoquinone), an antithrombotic agent, is mediated by thromboxane (TX) A2 receptor blockade with TXA2 synthase inhibition and suppression of cytosolic Ca2+ mobilization. J Pharmacol Exp Ther. 2005; 312:214-9.

10

Hubbard GP, Stevens JM, Cicmil M, Sage T, Jordan PA, Williams CM, et al. Quercetin inhibits collagen-stimulated platelet activation through inhibition of multiple components of the glycoprotein VI signaling pathway. J Thromb Haemost. 2003; 1:1079-88.

11

Ni H, Freedman J. Platelets in hemostasis and thrombosis: role of integrins and their ligands. Transfus Apher Sci. 2003; 28: 257-64.

12

Jin JL, Lee YY, Heo JE, Lee SH, Kim JM, Yun- Choi HS. Anti-platelet pentacyclic triterpenoids from leaves of Campsis grandiflora. Arch Pharm Res. 2004; 27:376-80.

13

Lee YY. Biological effects of components isolated from Angelica genuflexa [PhD thesis]. Seoul, Korea: Seoul National University.; 2004.

14

Heo J. Donguibogam. (1610) translated by Yoon SH, Kim HJ. Hadong: Donguibogam Press. 2005.

15

Armida PT, Quan SD, Jose Y, Sylvie AY, Adam KM. Inhibition of platelet aggregation in whole blood by alcohol. Thromb Res. 1995; 78:107-15.

16

Jeon WK, Lee JH, Kim HK, Lee AY, Lee SO, Kim YS, et al. Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes. J Ethnopharmacol. 2006; 106:62-69.

17

DiMinno G, Silver MJ. Mouse antithrombotic assay: A simple method for the evaluation of antithrombotic agents in in vivo potentiation of antithrombotic activity by ethyl alcohol. J Pharmacol Exp Ther. 1983; 225:57-60.

18

Makino T, Wakushima H, Okamoto T, Okukubo Y, Saito K, Kano Y. Effects of Kangen-karyu on coagulation system and platelet aggregation in mice. Biol Pharm Bull. 2003; 25:523-5.

19

Hubbard GP, Stevens JM, Cicmil M, Sage T, Jordan PA, Williams CM, et al. Quercetin inhibits collagen-stimulated platelet activation through inhibition of multiple components of the glycoprotein VI signaling pathway. J Thromb Haemost. 2003; 1:1079-88.

20

Wang RR. Dong Zhongshu's transformation of Yin-Yang theory and contesting of gender identity. Philo East & West. 2005; 55:209-31.

21

Liu Y, Dong L. (2002) Basic Theories of Traditional Chinese Medicine, Beijing: Academy Press. 2002:39.

22

Szeto YT, Benzie IF. Is the yin-yang nature of Chinese herbal medicine equivalent to antioxidationoxidation? J Ethnopharmacol. 2006; 108:361-6.

23

Ou B, Huang D, Hampsch-Woodill M, Flanagan JA. When east meets west: the relationship between yin-yang and antioxidation-oxidation. FASEB J. 2003; 17:127-9.

24

Ko KM, Leon TY, Mak DH, Chiu PY, Du Y, Poon MK. A characteristic pharmacological action of ‘Yang-invigorating’ Chinese tonifying herbs: enhancement of myocardial ATP-generation capacity. Phytomedicine. 2006; 13:636-42.

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