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- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Effects of Red Ginseng-Ejung-tang Water Extract on Cytokine Production in LPS-induced Mouse Macrophages
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2012, v.33 no.4, pp.42-49
(2012). Effects of Red Ginseng-Ejung-tang Water Extract on Cytokine Production in LPS-induced Mouse Macrophages. Journal of Korean Medicine, 33(4), 42-49.
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Abstract
Objectives: The purpose of this study was to investigate effects of Red Ginseng-Ejung-tang Water Extract (ER) on cytokine production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Methods: Levels of various cytokines such as interleukin (IL)-6, IL-10, IL-2, IL-12p70, vascular endothelial growth factor (VEGF), monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-2, keratinocyte-derived chemokine (KC), tumor necrosis factor (TNF)-alpha, granulocyte macrophage colony-stimulating factor (GM-CSF) were measured by high-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. Results: ER significantly decreased levels of IL-6, IL-10, IL-2, IL-12p70, VEGF, and MCP-1 for 24 hrs incubation at the concentrations of 25, 50, and 100 ㎍/mL in LPS-induced RAW 264.7 cells (P < 0.05). But ER did not exert significant effects on production of MIP-2, KC, TNF-α, and GM-CSF in LPS-induced RAW 264.7 cells. Conclusions: These results suggest that ER has an anti-inflammatory property related with its inhibition of cytokine production in LPS-induced macrophages.
- keywords
- Red ginseng, Ejung-tang, macrophage, inflammation, cytokine
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