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E-ISSN : 2713-8615

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Vol.10 No.1

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Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea

Journal of Species Research / Journal of Species Research, (E)2713-8615
2021, v.10 no.1, pp.1-11
Lee Jaeyun
Yun-Seok Jeong
KIM PIL SOO
Dong-Wook Hyun
Bae Jin-Woo
Lee, J. , Yun-Seok, J. , KIM, P. S. , Dong-Wook, H. , & Bae, J. (2021). Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea. Journal of Species Research, 10(1), 1-11.

Abstract

Evidence highlighting the importance of gut microbiota in biodiversity conservation is growing; however, gut bacteria in South Korean wildlife have not been well identified. Using a culture-dependent isolation method, we identified the gut bacteria from Korean aquatic wildlife: the gazami crab (Portunus trituberculatus), Korean striped bitterling (Acheilognathus yamatsutae), oily bitterling (Acheilognathus koreensis), leopard mandarin fish (Siniperca scherzeri), Korean dark chub (Zacco koreanus), diving beetle (Cybister lewisianus), spotted steed (Abbottina springeri), and Korean spotted sleeper (Odontobutis obscura interrupta). We identified 18 strains previously unrecorded in South Korea by comparing 16S rRNA gene sequences of isolates against the EzBioCloud and National Institute of Biological Resources (NIBR) databases. The isolated strains belong to the phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. We also assessed for phylogenetic relatedness, Gram-stain reaction, colony and cell morphology, and biochemical characteristics. Basic information and 16S rRNA gene sequences of the isolates were registered in NIBR, and NIBR accession numbers are provided.

keywords
16S rRNA gene sequences, gut microbiota, Korean aquatic wildlife, unidentified Korean gut bacteria

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