- KOREAN
- E-ISSN2713-8615
- KCI
E-ISSN : 2713-8615
A report on 17 unrecorded bacterial species in Korea isolated from Lakes Soyang and Chungju in 2016
JOUNG YO CHAN
Su Hyun Kim
Lim Yeonjung
JANGCHEON CHO
Abstract
As a part of the research program ‘Survey of freshwater organisms and specimen collection’, freshwater samples were collected from Lakes Soyang and Chungju in 2016. Hundreds of bacterial strains were isolated from the samples and were identified based on 16S rRNA gene sequences. Among the bacterial isolates, strains showing higher than 98.7% sequence similarity with validly published bacterial species not reported in Korea were selected as unrecorded bacterial species. Based on 16S rRNA gene sequence similarity, 17 strains were identified as unrecorded bacterial species in Korea. The 17 bacterial strains were phylogenetically diverse and belonged to four phyla, seven classes, 13 orders, 14 families, and 16 genera. At generic level, the unreported species were affiliated with Caulobacter, Paracoccus, and Mesorhizobium of the class Alphaproteobacteria, Deefgea, Undibacterium, Chitinimonas, Inhella, and Sphaerotilus of the class Betaproteobacteria, Vibrio and Cellvibrio of the class Gammaproteobacteria, Sanguibacter and Clavibacter of the phylum Actinobacteria, Lactococcus of the phylum Firmicutes, Deinococcus of the class Deinococci, and Chryseobacterium and Flavobacterium of the phylum Bacteroidetes. The unreported species were further characterized by examining Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic position. The detailed description of the 17 unreported species are also provided.
- keywords
- Freshwater, unreported bacterial species, Lake Soyang, Lake Chungju
Reference
Azam, F., T. Fenchel, J.G. Field, J.S. Gray, L.A. Meyer-Reil and F. Thingstad. 1983. The ecological role of water-column microbes in the sea. Mar. Ecol. Prog. Ser. 10(1):257-263.
Cotner, J.B. and B.A. Biddanda. 2002. Small players, large role: Microbial influence on biogeochemical processes in pelagic aquatic ecosystems. Ecosystems 5(2):105-121.
Felsenstein, J. 1985. Confidence-limits on phylogenies - an approach using the bootstrap. Evolution 39(4):783-791.
Jeon, Y.S., K. Lee, S.C. Park, B.S. Kim, Y.J. Cho, S.M. Ha and J. Chun. 2014. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int. J. Syst. Evol. Microbiol. 64(2):689-691.
Kara, E.L., P.C. Hanson, Y.H. Hu, L. Winslow and M.D. McMahon. 2013. A decade of seasonal dynamics and co-occurrences within freshwater bacterioplankton communities from eutrophic Lake Mendota, WI, USA. ISME J. 7(3):680-684.
Kim, O.S., Y.J. Cho, K. Lee, S.H. Yoon, M. Kim, H. Na, S.C. Park, Y.S. Jeon, J.H. Lee, H. Yi, S. Won and J. Chun. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62(3):716-721.
Kimura, M. 1983. Rare variant alleles in the light of the neutral theory. Mol. Biol. Evol. 1(1):84-93.
Lozupone, C.A. and R. Knight. 2007. Global patterns in bacterial diversity. Proc. Natl. Acad. Sci. U. S. A. 104:11436-11440.
Newton, R.J., S.E. Jones, A. Eiler, K.D. McMahon and S. Bertilsson. 2011. A guide to the natural history of freshwater lake bacteria. Microbiol. Mol. Biol. Rev. 75(1):14-49.
Rheinheimer, G. 1980. Aquatic microbiology. Wiley, New York.
Saitou, N. and M. Nei. 1987. The neighbor-joining method - a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4):406-425.
Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol. 30(12):2725-2729.
Wetzel, R.G. (2001). Limnology: lake and river ecosystems. Gulf Professional Publishing.
Williamson, C.E., W. Dodds, T.K. Kratz and M.A. Palmer. 2008. Lakes and streams as sentinels of environmental change in terrestrial and atmospheric processes. Fron. Ecol. Environ. 6(5):247-254.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations