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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • P-ISSN2234-7550
  • E-ISSN2234-5930
  • SCOPUS, KCI, ESCI

연조직 증강을 위한 세포치료 시 비계로서 키토산의 온도 감응성 및 섬유모세포의 증식에 미치는 영향에 관한 연구

STUDY ON THERMOSENSITIVITY OF CHITOSAN SCAFFOLD AND ON ITS EFFECTS ON FIBROBLAST PROLIFERATION IN CELL THERAPY FOR SOFT TISSUE AUGMENTATION

Journal of the Korean Association of Oral and Maxillofacial Surgeons / Journal of the Korean Association of Oral and Maxillofacial Surgeons, (P)2234-7550; (E)2234-5930
2009, v.35 no.3, pp.146-152
김정호 (서울대학교)
최진영 (서울대학교)

Abstract

Traditional surgical method or injection using filler is performed for soft tissue augmentation. Surgical methods have disadvantage of surgical morbidity. Commercially available injectable materials have the disadvantages such as resorption, short-term effect. repeated application and hypersensitivity. Significant shortcoming of cell therapy using autologous fibroblasts is delay of treatment effect. Chitosan/β-glycerol phosphate (GP) solution has thermosensitive property and allows sol-gel transition at physiologic pH and temperature. These properties may resolve the delay of treatment effect. The purposes of this study are to evaluate the viscosity and pH changes of chitosan/β-GP solutions and to evaluate the effect of chitosan/β-GP solution on fibroblast proliferation and production of collagen. We measured the viscosity and pH as function of temperature, of the solution containing 1:0.7, 1:0.75, 1:0.8 chitosan (1, 10, 100, 700 kDa) /β-GP. Fibroblasts from ears of 5 rats were cultured in chitosan/β-GP solutions for 3 weeks. Cell proliferation and collagen contents were measured every week with WST (water-soluble tetrazolium salt) assay and Collagen assay respectively. The Results are 1) Chitosan(100 kDa<)/β-GP solution (1:0.75) showed sol-gel transition at physiologic pH and body temperature and injectable properties. It will enable to resolve the delay in treatment effect 2) Cell proliferation and total collagen contents of the control group were increased with time. However, these decreased after the 1st week in experimental group 3) Collagen contents in the experimental group are higher than that of control group. Chitosan/β-GP solution may provide favorable conditions for cell function

keywords
Soft tissue augmentation, Chitosan/β-glycerol phosphate, Fibroblast, Thermosensitive, Scaffold

Journal of the Korean Association of Oral and Maxillofacial Surgeons