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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
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ISSN : 0376-4672

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59권 11호

JD005 화합물이 인간치수유래줄기세포의 상아질 모세포 분화에 미치는 영향에 관한 연구

The effect of JD005 compound on odontogenic differentiation of human dental pulp stem cells

김다솔 (부산대학교)
이남걸 (제이디바이오사이언스 기업부설연구소)
장혜옥 (부산대학교)
안진희 (광주과학기술원 화학과)
김형룡 (전북대학교 치과대학 약리학교실)
대한치과의사협회지 / The Journal of the Korean Dental Association, (P)0376-4672; (E)2713-7961
2021, v.59 no.11, pp.640-648
https://doi.org/10.22974/jkda.2021.59.11.003
김다솔, 이남걸, 장혜옥, 안진희, & 김형룡. (2021). JD005 화합물이 인간치수유래줄기세포의 상아질 모세포 분화에 미치는 영향에 관한 연구. 대한치과의사협회지, 59(11), 640-648, https://doi.org/10.22974/jkda.2021.59.11.003

초록

치수유래줄기세포는 중간엽 줄기세포로 다른 종류의 줄기세포와 마찬가지로 self-renewal 하고, 주어진 환경에서 다양한 세포로 분화가 가능한 특징을 가지고 있다. 이러한 분화의 특성은 자연치유가 힘든 치아경조직의 재생을 유도하며, 이러한 결과는 자연치아의 보존에 중요한 역할을 할 수 있을 것으로 사료된다. 본 연구에서는 ZM241385를 참고하여 JD005 화합물을 합성하였으며, 이를 이용하여 치수유래줄기세포를 상아질 모세포로 분화 유도 하면서 화합물을 처리 하고, 상아질 모세포 분화 유도 시 어떠한 변화가 있는지 확인 해 보았다. 본 연구에서 JD005는 상아질 모세포 분화를 촉진시키는 것으로 나타났으며 분화 마커인 ALP, RUNX2, OPN, DSPP 및 DMP1은 JD005 처리 시 크게 증가하였다. 위와 관련된 세포신호전달인자인 SMAD의 인산화도 JD005을 처리한 세포에서 크게 증가하였으며 JD005가 상아모세포 분화를 통한 상아질 재생에 기여할 수 있음을 시사하였다.

keywords
DPSCs, JD005, dentine regeneration, odontoblasts, RUNX2, DSPP, SMAD phosphorylation

Abstract

Preservation of natural teeth and their structure is critical for chewing, swallowing, and better speech. Dentin and dental tissues are crucial in maintaining natural teeth and their structure, but treatment of dental tissue and dentin fracture is challenging for dentistry. Hence, scientific understanding needs to be translated to discover novel dental treatments. Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) isolated from the third molar pulp. These cells have similar characteristics to MSCs with a unique property where DPSCs can differentiate into dentin forming odontoblast-like cells. Here, we made an effort to evaluate the potential substrate that induces odontoblastic differentiation of DPSCs. In this study, JD005, a modified A2A antagonist from ZM-241385, was determined to enhance OD. Specifically, JD005 treatment significantly enhanced the OD markers like alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), OPN, dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1). Additionally, the DPSCs treated with JD005 showed enhanced SMAD phosphorylation associated with differentiation. These observations distinctively suggest that JD005 potentially contributes to dentin regeneration via odontoblast differentiation.

keywords
DPSCs, JD005, dentine regeneration, odontoblasts, RUNX2, DSPP, SMAD phosphorylation

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