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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

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人蔘 사포닌을 비롯한 界面活性劑가 胃腸管內의 蛋白質 加水分解酵素 反應에 미치는 影響

The effects of surfactants including ginseng saponins on the gastric enzyme-catalyzed hydrolysis

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2006, v.27 no.2, pp.103-111
김영재 (창원대학교 자연과학대학원)
이상직 (영남대학교)
박기태 (박기태한의원)
김영재, 이상직, & 박기태. (2006). 人蔘 사포닌을 비롯한 界面活性劑가 胃腸管內의 蛋白質 加水分解酵素 反應에 미치는 影響. 대한한의학회지, 27(2), 103-111.
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Abstract

Objectives : This study was conducted to investigate the effects of ginseng saponins and commercial surfactants such as Triton X-100, sodium deoxycholate, and sodium dodecyl sulfate on the gastric enzyme-catalyzed hydrolysis. Methods : Saponins (a surface-active plant component) from fresh ginseng root were extracted to examine its effect on the gastric enzyme-catalyzed hydrolysis. Commercial surfactants such as Triton X-100, sodium deoxycholate, and sodium dodecyl sulfate were also employed in the hydrolysis system to compare their effects with that of the ginseng saponins. The effects of surfactants on the gastric enzyme-catalyzed hydrolysis were measured by using spectrophotometer. Spectropolarimeter was used to examine the conformational change of enzymes and substrates by the addition of ginseng saponins into the system. Results : Both the tryptic and the peptic digestion of milk casein or egg albumin were slightly improved with an increase in the amount of ginseng saponins in the system. Triton X-100 showed an effect similar to that of ginseng saponins, while sodium dodecyl sulfate and sodium deoxycholate diminished the hydrolysis. Circular dichroism spectra of enzymes and substrates was significantly changed by the addition of ginseng saponins into the system. Conclusions : These results showed that ginseng saponins affected positively the gastric enzyme-catalyzed hydrolysis, and suggested that the digestion of substrates by gastric enzymes was affected by the change of enzyme conformation by ginseng saponins.

keywords
Ginseng saponins, surfactants, pepsin, trypsin


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