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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

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아밀로이드 베타 단백질에 의해 유도된 신경세포 독성에 대한 원추리의 억제 효과 탐색

Protective effects of Hemerocallis fulva extracts on amyloid β-protein-induced death in neuronal cells

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2006, v.27 no.2, pp.122-133
김은숙 (창원대학교 자연과학대학)
최수진 (경성대학교)
류병호 (경성대학교)
최진호 (부경대학교)
오명석 (광주과학기술원 생명과학과)
박우진 (광주과학기술원)
최영환 (부산대학교)
백도현 ((주)네오허브 한밭생명공학연구소)
하권철 ((주)네오허브 한밭생명공학연구소)
강대욱 ((주)네오허브 한밭생명공학연구소)
조용권 (창원대학교)
박기태 ((주)네오허브 한밭생명공학연구소)
문자영 (창원대학교)
김은숙, 최수진, 류병호, 최진호, 오명석, 박우진, 최영환, 백도현, 하권철, 강대욱, 조용권, 박기태, & 문자영. (2006). 아밀로이드 베타 단백질에 의해 유도된 신경세포 독성에 대한 원추리의 억제 효과 탐색. 대한한의학회지, 27(2), 122-133.
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Abstract

Objectives: The amyloid β-protein (Aβ) is the principal component of the senile plaques characteristic of Alzheimer’s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify Aβ toxicity. In this study, we have investigated the protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on Aβ-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. Methods: For this study, we used MTT reduction assay for detection of protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on Aβ25-35-induced cytotoxicity to PC12 cells. We also used cell-based β-secretase assay system to investigate the inhibitory effect of water- and organic solvent-extracts of Hemerocallis fulva root on β-secretase activity. Results: We previously reported that methanol extracts of Hemerocallis fulva root strongly attenuated cytotoxicity induced by the three Aβ fragments (Aβ25-35, Aβ1-42 Aβ1-43) to both SK-N-MC and PC12 cells. In the present study, we found that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol had the strong protective effects against Aβ25-35-induced cytotoxicity to PC12 cells and inhibitory potency to β-secretase activity. Conclusion: These results suggest that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol may contain the protective component(s) against Aβ-induced cell death in PC12 cells as well as inhibitory component(s) to β-secretase activity.

keywords
amyloid β-protein, BACE, β-secretase, Hemerocallis fulva, Liliaceae, PC12 cell, amyloid β-protein, BACE, β-secretase, Hemerocallis fulva, Liliaceae, PC12 cell


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