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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Protective Effect of Red Ginseng and Paeonia radix against Nitric Oxide-Induced Apoptosis in Human Neuroblastoma SK-N-MC cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2007, v.28 no.1, pp.198-210



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Abstract

Objectives Nitric oxide (NO) is a reactive free radical and a messenger molecule in many physiological functions. However, excessive release NO of induces neurotoxicity. We investigated whether a mixture of red ginseng and paeonia radix possesses a protective effect against sodium nitroprusside (SNP)-induced apoptosis in the human neuroblastoma cell line SK-N-MC. Methods We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme activity assay in SK-N-MC cells. Results MTT assay showed that SNP treatment significantly reduced the viabilities of cells and that pre-treatment with the red ginseng and paeonia radix mixture alleviated SNP-induced cytotoxicity. The cells treated with SNP exhibited several apoptotic features, while those pre-treated for 1 h with the mixture of red ginseng and paeonia radix for 1 h prior to SNP expose showed reduced apoptotic features. In addition, the cells pre-treated with the red ginseng and paeonia radix mixture for 1 h prior to SNP expose increased bcl-2 expressions, decreased Bax expressions, and decreased caspase-3 enzyme activity. Conclusions These results show that the red ginseng and paeonia radix mixture exerts a protective effect against SNP-induced apoptosis in SK-N-MC cells.

keywords
Nitric oxide, apoptosis, SK-N-MC cells, red ginseng, paeonia radix cels, red ginseng, paeonia radix


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