ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
사전등록 바로가기
 

논문 상세

Home > 논문 상세
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

이전

다음

45권 3호
Share

漆皮가 급성림프구성백혈병 세포주 MOLT-4의 세포자멸과 자가포식에 미치는 영향

The Effect of Rhus verniciflua Stokes Extract on Apoptosis and Autophagy in human leukemic cell line (MOLT-4)

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2024, v.45 no.3, pp.154-167
이주영 (경희대학교 한의과대학 간계내과학교실)
이장훈 (경희대학교 한의과대학 간계내과학교실)
김영철 (경희대학교 한의과대학 간계내과학교실)
이주영, 이장훈, & 김영철. (2024). 漆皮가 급성림프구성백혈병 세포주 MOLT-4의 세포자멸과 자가포식에 미치는 영향. 대한한의학회지, 45(3), 154-167.
  • 다운로드 수
  • 조회수

Abstract

Objectives: This study was performed to investigate the effects of Rhus verniciflua Stokes (RVS) on apoptosis and autophagy in the human leukemic cell line, MOLT-4. Methods: Cell viability was measured by MTS/PMS assay, and cell cycle distribution was analyzed by flow cytometry. The expression levels of mRNA implicated in apoptosis and ER-stress were investigated with RT-qPCR. Lastly, apoptosis- and autophagy-related protein expressions were measured by Western blot analysis. Results: RVS inhibited proliferation of MOLT-4 in a dose-dependent manner over 24, 48 and 72 hours. RVS treatment also induced an increase in subG1 phase. Exposure to RVS increased the expression of the mRNAs encoding Bax and caspase-3, while decreasing the expression of Bcl-2 mRNA, suggesting that RVS induced apoptosis in MOLT-4 cells. Additionally, RVS extract up-regulated ER-stress related mRNAs such as IRE1α, CHOP, PERK and ATF6. Changes in RVS extract-induced apoptosis and autophagy proteins on MOLT-4 cells were also investigated. The level of Bcl-2 was decreased, whereas the levels of Bax, caspase-3, AMPK, Beclin-1, Atg5, p62, and LC3II were increased. Conclusion: These results suggest that RVS would be beneficial in the treatment of Acute Lymphoblastic Leukemia.

keywords
Rhus verniciflua Stokes, Rhois Vernicifluae Cortex, MOLT-4, Acute Lymphoblastic Leukemia, Apoptosis, Autophagy


  • 다운로드 수
  • 조회수
  • 0KCI 피인용수
  • 0WOS 피인용수

권호 내 다른 논문

추천 논문

상단으로 이동

권호 목록

소개

규정 및 지침

게시판

대한한의학회지

  학술지 홈페이지 이용 환경은   크롬 브라우저 사용을 권장합니다.

대한한의학회지

논문 투고 OA 정책