Journal of Species Research, 국립생물자원관

PDF Citaion Description of 41 unrecorded bacterial species in Korea, isolated from freshwater in 2021

임완택(한경국립대학교) pp.3-16 https://doi.org/10.12651/JSR.2024.13.4.353

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Here we describe indigenous prokaryotic species in Korea, a total of 41 bacterial strains were isolated from freshwater from the Republic of Korea. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to predefined bacterial species. There is no official report that these 41 species belonged to 5 phyla, 10 classes, 18 orders, 23 families, and 29 genera, which were assigned to Streptomyces, Mycolicibacterium, Smaragdicoccus, Nocardiopsis, and Nocardia of the phylum Actinobacteria; Runella, Flavobacterium, Algoriphagus, Sphingobacterium, and Aequorivita of the phylum Bacteroidota; Paenibacillus, Bacillus, Metabacillus, and Fredinandcohnia of the phylum Firmicutes; Sphingobium, Erythrobacter, Duganella, Methylopila, Novosphingobium, Azospirillum, Simplicispira, Corallococcus, Pseudomonas, Devosia, Pseudorhodoferax, Pseudomonas, Prolinoborus, Pectobacterium, and Aquabacterium of the phylum Proteobacteria; Proshecobacter of the phylum Verrucomicrobia. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

PDF Citaion Three species of the subgenus Bessobates Townes, Townes & Gupta of Netelia Gray (Hymenoptera: Ichneumonidae: Tryphoninae) new to South Korea

최진경(대구교육대학교) pp.17-22 https://doi.org/10.12651/JSR.2024.13.4.36

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Three newly recorded species of the subgenus Bessobates of Netelia Gray, 1860 are reported. Three species, Netelia (Bessobates) longipad, N. (B.) pallescens and N. (B.) yakushimensis, are recorded for the first time from South Korea. Photographs and diagnoses of three unrecorded species of this subgenus are provided.

PDF Citaion New record of two spirotrichous ciliate species (Protozoa, Ciliophora) collected from coasts of South Korea

정재호(강릉원주대학교) pp.23-27 https://doi.org/10.12651/JSR.2024.13.4.373

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Two newly recorded ciliates, Aspidisca major (Madsen, 1931) Kahl, 1932 and Metaurostylopsis rubra Song and Wilbert, 2002, were collected from eastern and southern coasts of South Korea, respectively. The morphology of these two species were studied based on observations of protargol-stained specimens. The morphological characteristics of the two species are as follows: (1) Aspidisca major is characterized by a size of 76-78×42-49 μm after protargol impregnation, two distinct macronuclei, seven frontoventral cirri in “polystyla-arrangement”, and dorsal kinety 1 extends to posterior end of dorsal kinety 2; and (2) Metaurostylopsis rubra is characterized by a size of 68-77×49-58 μm size after protargol impregnation, brick-reddish body color, and large number of marginal rows. In the present study, we provide a brief diagnosis, remarks, and photomicrographs.

PDF Citaion Report of five unrecorded bacterial species in Korea belonging to the genus Bradyrhizobium

허준(국립농업과학원 농업미생물과) pp.28-34 https://doi.org/10.12651/JSR.2024.13.4.378

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Symbiotic bacteria belonging to the genus Bradyrhizobium were generally called ‘rhizobia’ which can fix nitrogen by nodulating legumes. Between 2000 and 2001, numerous Bradyrhizobium strains were isolated from root nodules. However, due to challenges in identification, many of these isolates remained unreported. Recent advances in phylogenetic analyses based on whole genome sequencing have resolved these difficulties in species identification. Consequently, five of these strains have now been re-identified and are described as previously unrecorded species in the Republic of Korea. As a result, we report Bradyrhizobium elkanii Glm-3 (=KACC 10989), Bradyrhizobium australafricanum Glm-4 (=KACC 10990), Bradyrhizobium huanghuaihaiense Glm-7 (=KACC 10993), these were isolated from root nodules of Glycine max, Bradyrhizobium frederickii Kus-5 (=KACC 11016) isolated from root nodules of Kummerowia striata and Bradyrhizobium diazoefficiens Leb-14 (=KACC 11026) isolated from root nodules of Lespedeza bicolor.

PDF Citaion Characterization of two unrecorded yeast species, Starmerella apicola and Symmetrospora symmetrica, isolated from Apis mellifera and Citrus sunki in Republic of Korea

김명겸(서울여자대학교) pp.35-40 https://doi.org/10.12651/JSR.2024.13.4.385

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The purpose of this study is to isolate and identify wild yeasts from Apis mellifera and Citrus sunki samples collected in Okcheon-gun and Jeju-si, Republic of Korea. Among ten strains, one strain had been previously reported, but nine strains were unreported in the Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were conducted using the Basic Local Alignment Search Tool (BLAST) (Fell et al., 2000). Cell morphologies were observed using a phase contrast microscope, and assimilation tests were performed using the API 20C AUX kit. The two unrecorded yeast strains, MBEE-1 and MMD-1, belong to the genus Starmerella (family Saccharomycetales incertae sedis, Order Saccharomycetales, Class Saccharomycetes) and the genus Symmetrospora (family Symmetrosporaceae, Order Cystobasidiomycetes incertae sedis, Class Cystobasidiomycetes), respectively. Both strains had oval-shaped and polar budding cells. This research elucidated the morphological and biochemical properties of these two previously unreported yeast species in Korea.

PDF Citaion A report of 7 unrecorded haloarchaea species from seawater samples near Dokdo island, Korea

서명지(인천대학교) pp.41-47 https://doi.org/10.12651/JSR.2024.13.4.391

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In July 2023, the seawater samples were collected near Dokdo island to obtain unrecorded haloarchaea in Korea. These samples were suspended in a 20% NaCl (w/v) solution and enriched for one month at 37℃ in fresh DB Characterization media No. 2 broth with simply modification. Further, the enriched culture was spread on agar medium to obtain single colonies of halophilic archaea. Phylogenetic analysis based on the 16S rRNA gene showed that the isolates from this study exhibited minimum 98.7% sequence similarity with previously reported species. Finally, 7 haloarchaeal species, which had not been reported in Korea but were validly published under the International Code of Nomenclature of Prokaryotes (ICNP), were obtained. These isolates were classified into the orders Halobacteriales. The 7 Halobacteriales species were further categorized into the family Haloferacaceae, comprising 2 genus, Haloferax and Haloplanus. Collectively, these unrecorded haloarchaeal species spanned 1 order, 1 family, and 2 genera. This research highlights the potential for discovering previously unknown species in domestic seawater environment. Comprehensive analyses, including Gram staining, cell morphology, physiological and basic biochemical parameters, and phylogenetic analysis, were conducted and are detailed for each species.

PDF Citaion Isolation and characterization of two unreported yeast species from wildflowers and mountain soil in Korea in the families Filobasidiaceae and Piskurozymaceae

Sathiyaraj Srinivasan(서울여자대학교) pp.48-53 https://doi.org/10.12651/JSR.2024.13.4.398

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The purpose of this study was to isolate and identify wild yeasts from wildflower samples in Okcheongun and soil samples in Nowon-gu, Republic of Korea. Out of the fourteen strains identified, twelve had been previously reported, whereas two strains were unreported in the Republic of Korea. Identification of wild yeast strains was performed through pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene using the Basic Local Alignment Search Tool (BLAST) (Fell et al., 2000). Cell morphology was examined using a phase contrast microscope, and assimilation tests were conducted with the API 20C AUX kit. All strains were classified under the phylum Basidiomycota. The two unreported yeast strains, MFL-1 and NYB3-16, are classified under the genus Naganishia (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes) and the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes), respectively. These two strains exhibited morphologies consistent with their type species. MFL-1 cells were circular, while NYB3-16 cells were oval. Both strains demonstrated polar budding. This study highlighted the morphological and biochemical characteristics of the two unreported yeast species that had not been previously documented in Korea. Keywords: 26S rRNA, Naganishia, Solicoccozyma,

PDF Citaion List of insect collection by Du-Myung Seok preserved at Kyushu University in Japan

안능호(국립생물자원관) pp.54-67 https://doi.org/10.12651/JSR.2024.13.4.404

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This report lists and illustrates 35 insect species from a collection of 129 specimens gathered by D. M. Seok, the first Korean entomologist. Most of Seok̓s collections were preserved at Kyushu University in Japan for about 90 years before being relocated to NIBR (National Institute of Biological Resources) in Korea in September 2024.

PDF Citaion New record of marine scuticociliates and euplotids (Protozoa, Ciliophora) from coastal waters of South Korea

정재호(강릉원주대학교) pp.67-73 https://doi.org/10.12651/JSR.2024.13.4.417

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During a field survey of marine ciliate diversity in South Korea, four ciliate species were newly recorded as follows: Falcicyclidium citriforme, Homalogastra setosa, Euplotes orientalis, and Euplotes shini. The identification of these species was based on morphological examination through observations of both living cells and silver-impregnated specimens. A brief description, remarks, and photomicrographs are provided for each species.

PDF Citaion Taxonomic review of Korean Ephemera Linnaeus with lectotype designation of E. sachalinensis Matsumura (Insecta: Ephemeroptera: Ephemeridae)

배연재(고려대학교) pp.73-83 https://doi.org/10.12651/JSR.2024.13.4.423

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The common mayfly genus Ephemera Linnaeus from South Korea was taxonomically reviewed, with emphasis on the sympatric E. orientalis McLachlan and E. sachalinensis Matsumura. A taxonomic key to South Korean Ephemera species is provided for larval and adult stages. The thick and continued pair of dark stripes on mesonotum and presence of pigmentation on abdominal tergum I-II are the diagnostic characters to distinguish E. orientalis from E. sachalinensis in both male and female adults and larvae. In the larvae, the shape and length of foreleg claws, and the shape of posterior margin of tergum X are useful diagnostic characters to distinguish between these two species. In addition, a female specimen deposited in the Hokkaido University Museum, Sapporo, Japan, that was used in the original description of E. sachalinensis by Matsumura in 1911 is designated as the lectotype of the species. A DNA barcoding analysis on the East Asian Ephemera species with paired multi-stripes on the abdominal terga well substantiated their species status.

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