Abstract

In the course of our survey of microbial diversity, a total of seven basidiomycetous yeast strains were isolated from flowers sampled across the four provinces of the Republic of Korea, of which two strains were identified as an unrecorded species. Comparative analysis was conducted for identification of strains using the D1/D2 region of the large subunit (LSU) rRNA gene sequences, observation of cell morphologies, and carbon assimilation tests. Seven strains were classified within the phylum Basidiomycota. Two unrecorded strains, JRP1-10 (KACC 410703) and KC-1 (KACC 410704), were identified as Vishniacozyma pseudocarnescens, belonging to the family Bulleribasidiaceae, order Tremellales, class Tremellomycetes. Two strains had ellipsoidal-shaped and monopolar budding cells and smooth and cream-colored colonies after cultivation at 25℃ for 3 days. Here, we present the morphological and biochemical characteristics of the unrecorded yeast species, Vishniacozyma pseudocarnescens, in the Republic of Korea.