- KOREAN
- E-ISSN2713-8615
- KCI
E-ISSN : 2713-8615
The first record of nine bacterial species belonging to the phylum Proteobacteria in Korea
kim juyoung
Myung Kyum Kim
Abstract
As part of a larger study with the aim to discover indigenous prokaryotic species in Korea, nine bacterial strains were isolated and assigned to the phylum Proteobacteria in 2016. High 16S rRNA gene sequence similarity (>98.5%) and formation of a robust phylogenetic clades with known species indicated that each strain belongs to an independent and predefined bacterial species. This is the first report of these nine species in Korea: two strains of the Methylobacterium, two strains of the Microvirga, one strain of the Pantoea, and four strains of the Psychrobacter, all within the Proteobacteria. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.
- keywords
- 16S rRNA, bacterial diversity, Proteobacteria, unreported species
Reference
Ardley, J.K., M.A. Parker, S.E. De Meyer, R.D. Trengove, G.W. O’Hara, W.G. Reeve, R.J. Yates, M.J. Dilworth, A. Willems and J.G. Howieson. 2012. Microvirga lupini sp. nov., Microvirga lotononidis sp. nov. and Microvirga zambiensis sp. nov. are alphaproteobacterial root-nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts. Int. J. Syst. Evol. Microbiol. 62:2579-2588.
Bakermans, C., H.L. Ayala-del-Río, M.A. Ponder, T. Vishnivetskaya, D. Gilichinsky, M.F. Thomashow and J.M. Tiedje. 2006. Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost. Int. J. Syst. Evol. Microbiol. 56:1285-1291.
Bowman, J.P., J. Cavanagh, J.J. Austin and K. Sanderson. 1996. Novel Psychrobacter species from Antarctic ornithogenic soils. Int. J. Syst. Evol. Microbiol. 46:841-848.
Brady, C.L., I. Cleenwerck, S.N. Venter, K. Engelbeen, P. De Vos and T.A. Coutinho. 2010. Emended description of the genus Pantoea, description of four species from human clinical samples, Pantoea septica sp. nov., Pantoea eucrina sp. nov., Pantoea brenneri sp. nov. and Pantoea conspicua sp. nov., and transfer of Pectobacterium cypripedii (Hori 1911) Brenner et al. 1973 emend. Hauben et al. 1998 to the genus as Pantoea cypripedii comb. nov. Int. J. Syst. Evol. Microbiol. 60:2430-2440.
Cao, Y.-R., Q. Wang, R.X. Jin, S.K. Tang, Y. Jiang, W.X. He, H.X. Lai, L.H. Xu and C.L. Jiang. 2011. Methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil. Antonie Leeuwenhoek 99:629-634.
Doetsch, R. 1981. Determinative methods of light microscopy. Manual of Methods for General Bacteriology: 21-33.
Edgar, R.C. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32:1792-1797.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
Gallego, V., M.T. García and A. Ventosa. 2005. Methylobacterium hispanicum sp. nov. and Methylobacterium aquaticum sp. nov., isolated from drinking water. Int. J. Syst. Evol. Microbiol. 55:281-287.
Hall, T.A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series, 1999. vol 41. [London]: Information Retrieval Ltd., c1979-c2000., pp 95-98.
Kanso, S. and B.K. Patel. 2003. Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int. J. Syst. Evol. Microbiol. 53:401-406.
Kim, O.-S., Y.J. Cho, K. Lee, S.H. Yoon, M. Kim, H. Na, S.C. Park, Y.S. Jeon, J.H. Lee, H. Yi, S. Won and J. Chun. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62:716-721.
Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
Romanenko, L.A., A.M. Lysenko, M. Rohde, V.V. Mikhailov and E. Stackebrandt. 2004. Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int. J. Syst. Evol. Microbiol. 54:1741-1745.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods Mol. Biol. Evol. 28:2731-2739.
Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703.
Williams, K.P., J.J. Gillespie, B.W. Sobral, E.K. Nordberg, E.E. Snyder, J.M. Shallom and A.W. Dickerman. 2010. Phylogeny of gammaproteobacteria. J. Bacteriol. 192:2305-2314.
Williams, K.P., B.W. Sobral and A.W. Dickerman. 2007. A robust species tree for the alphaproteobacteria. J. Bacteriol. 189:4578-4586.
Woese, C.R. 1987. Bacterial evolution. Microbiological reviews 51:221-271.
Wragg, P., L. Randall and A. Whatmore. 2014. Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest. J. Microbiol. Methods 105:16-21.
Yumoto, I., K. Hirota, Y. Sogabe, Y. Nodasaka, Y. Yokota and T. Hoshino. 2003. Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int. J. Syst. Evol. Microbiol. 53:1985-1989.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations