- KOREAN
- E-ISSN2713-8615
- KCI
E-ISSN : 2713-8615
Isolation of wild yeasts from soils collected in Pochoen-si, Korea and characterization of unrecorded yeasts
Yuna Park
Sathiyaraj Srinivasan (Seoul Women’s University)
Abstract
In 2019, as a subset study to discover indigenous yeast species in Korea, a total of 20 yeast species were isolated from soil samples collected in Pochoen-si. Among them, eight strains were unreported species. From the high 26S rRNA gene sequence similarity and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to independent and predefined yeast species. The 20 strains were assigned to the genera Aureobasidium (1 strain) and Meyerozyma (1 strain) of the phylum Ascomycota and Cystofilobasidium (2 strains), Filobasidium (1 strain), Naganishia (2 strains), Bullera (3 strains), Leucosporidium (9 strains) and Sampaiozyma (1 strain) of the phylum Basidiomycota. There is no official report of the following species in Korea: Leucosporidium creatinivorum (4 strains), Leucosporidium escuderoi (2 strains), Leucosporidium golubevii (1 strain) and Leucosporidium intermedium (2 strains). Basic biochemical characteristics, colony and cell morphology are also described in the species description section.
- keywords
- 26S rRNA, Leucosporidium, unreported species, yeast diversity
Reference
Carrasco, M., J.M. Rozas, S. Barahona, J. Alcaíno, V. Cifuentes and M. Baeza. 2012. Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC Microbiol 12:251.
Di Menna, M.E. 1960. Yeasts from Antarctica. J Gen Microbiol 23:295-300.
Di Menna, M.E. 1966. Yeasts in Antarctic soils. Antonie Van Leeuwenhoek 32:29-38.
Duarte, A.W.F., I. Dayo-Owoyemi, F.S. Nobre, F.C. Pagnocca, L.C.S. Chaud, A. Pessoa, M.G.A. Felipe and L.D. Sette. 2013. Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples. Extremophiles 17:1023-1035.
Fell, J.W., A.C. Statzell, I.L. Hunter and H.J. Phaff. 1969. Leucosporidium gen. n., the heterobasidiomycetous stage of several yeasts of the genus Candida. Antonie Van Leeuwenhoek 35:433-462.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
Goto, S., J. Sugiyama and H. Lizuka. 1969. A taxonomic study of Antarctic yeasts. Mycologia 61:748-774.
Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datadets. Molecular Biology and Evolution 33:1870-1874.
Kurtzman, C.P. and C.J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73(4):331-371.
Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
Sampaio, J.P. 2011a. Leucosporidiella Sampaio 2003. In: C.P. Kurtzman, J.W. Fell, T. Boekhout (eds.), The yeasts, a taxonomic study, vol 3, 5th edn. Elsevier, New York, pp. 1801-1806.
Sampaio, J.P. 2011b. Leucosporidium Fell, Statzell, Hunter and Phaff 1969. In: C.P. Kurtzman, J.W. Fell, T. Boekhout (eds.), The yeasts, a taxonomic study, vol 3, 5th edn. Elsevier, New York, pp. 1485-1494.
Sampaio, J.P., M. Gadanho, R. Bauer and M. Weiß. 2003. Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales. Mycol Prog 2:53-68.
Sampaio, J.P., W.I. Golubev, J.W. Fell, M. Gadanho and N.W. Golubev. 2004. Curvibasidium cygneicollum gen. nov., sp. nov. and Curvibasidium pallidicorallinum sp. nov., novel taxa in the Microbotryomycetidae (Urediniomycetes), and their relationship with Rhodotorula fujisanensis and Rhodotorula nothofagi. Int J Syst Evol Microbiol 54:1401-1407.
Vaca, I., C. Faúndez, F. Maza, B. Paillavil, V. Hernández, F. Acosta, G. Levicán, C. Martínez and R. Chávez. 2013. Cultivable psychrotolerant yeasts associated with Antarctic marine sponges. World J Microbiol Biotechnol 29:183-189.
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