- KOREAN
- E-ISSN2713-8615
- KCI
E-ISSN : 2713-8615
Eight unrecorded bacterial species isolated from soil and marine sediment in Korea
Ki-Eun Lee
In-Tae Cha (Microorganism Resources Division, National Institute of Biological Resources, Republi)
Byoung-Hee Lee
Park， Soo Je
Abstract
The Earth contains billions of microbial species, although the vast majority cannot be cultured in laboratories and are thus considered unidentified and uncharacterized. Extremophiles are microorganisms that thrive in extreme conditions, including temperature, salinity, and pH. Extremophilic microorganisms have provided important insights for biological, metabolic, and evolutionary studies. Between 2017 and 2019, as part of a comprehensive investigation to identify bacterial species in Korea, eight bacterial strains were isolated from marine and non-marine environments in Jeju Island. These strains were cultured under extreme salinity or pH conditions. Phylogenetic analysis using 16S ribosomal RNA (rRNA) gene sequencing indicated that all eight strains belonged to the phyla Gammaproteobacteria, Bacilli, and Alphaproteobacteria. Based on their high 16S rRNA gene sequence similarities (>98.7%) and the formation of strong monophyletic clades with their closest related species, all isolated strains were considered as an unrecorded strain, previously unidentified species. Gram stain reaction, culture conditions, colony and cell morphology, biochemical characteristics, isolation source, and National Institute of Biological Resources (NIBR) IDs are described in this article. The characterization of these unrecorded strains provides information on microorganisms living in Korea.
- keywords
- 16S rRNA gene sequence, soil, marine, unrecorded species
Reference
Coker, J.A. 2016. Extremophiles and biotechnology: Current uses and prospects. F1000Res. 5.
Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution. 39:783-791.
Kim, O.S., Y.J. Cho, K. Lee, S.H. Yoon, M. Kim, H. Na, S.C. Park, Y.S. Jeon, J.H. Lee, H. Yi, S. Won and J. Chun. 2012. Introducing eztaxon-e: A prokaryotic 16s rrna gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. 62:716-721.
Kumar, S., G. Stecher and K. Tamura. 2016. Mega7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33:1870-1874.
Locey, K.J. and J.T. Lennon. 2016. Scaling laws predict global microbial diversity. Proc Natl Acad Sci U S A. 113:5970-5975.
Merino, N., H.S. Aronson, D.P. Bojanova, J. Feyhl-Buska, M.L. Wong, S. Zhang and D. Giovannelli. 2019. Living at the extremes: Extremophiles and the limits of life in a planetary context. Front Microbiol. 10:780.
Overmann, J. and A.H. Scholz. 2017. Microbiological research under the nagoya protocol: Facts and fiction. Trends Microbiol. 25:85-88.
Pikuta, E.V., R.B. Hoover and J. Tang. 2007. Microbial extremophiles at the limits of life. Crit Rev Microbiol. 33:183-209.
Rothschild, L.J. and R.L. Mancinelli. 2001. Life in extreme environments. Nature. 409:1092-1101.
Rourke, M.F. 2018. Who are indigenous and local communities and what is traditional knowledge for virus access and benefit-sharing? A textual analysis of the convention on biological diversity and its nagoya protocol. J Law Med. 25:707-726.
Saitou, N. and M. Nei. 1987. The neighbor-joining method:A new method for reconstructing phylogenetic trees. Mol Biol Evol. 4:406-425.
Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The clustal_x windows interface:Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882.
Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J. Lane. 1991. 16s ribosomal DNA amplification for phylogenetic study. J Bacteriol. 173:697-703.
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