ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Statistical Characterization of the Multi-Charged Fragment Ions in the CID and HCD Spectrum

Statistical Characterization of the Multi-Charged Fragment Ions in the CID and HCD Spectrum

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.2, pp.41-46
https://doi.org/10.5478/MSL.2021.12.2.41
Sangeetha Ramachandran (Department of Electronics, Cochin University of Science and Technology)
Tessamma Thomas (Department of Electronics, Cochin University of Science and Technology)
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Abstract

Collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD) are the widely used fragmenta-tion technique in mass spectrometry-based proteomics studies. Understanding the fragmentation pattern from the tandem mass spectra using statistical methods helps to implement efficient spectrum analysis algorithms. The study characterizes the frequency of occurrence of multi-charged fragment ions and their neutral loss events of doubly and triply charged peptides in the CID and HCD spectrum. The dependency of the length of the fragment ion on the occurrence of multi-charged fragment ion is characterized here. Study shows that the singly charged fragment ions are generally dominated in the doubly charged peptide spectrum. However, as the length of the product ion increases, the frequency of occurrence of charge 2 fragment ions increases. The y- ions have more tendencies to generate charge 2 fragment ions than b- ions, both in CID and HCD spectrum. The frequency of occurrence of charge 2 fragment ion peaks is prominent upon the dissociation of the triply charged peptides. For triply charged peptides, product ion of higher length occurred in multiple charge states in CID spectrum. The neutral loss peaks mostly exist in charge 2 states in the triply charged peptide spectrum. The b-ions peaks are observed in much less frequency than y-ions in HCD spectrum as the length of the fragment increases. Isotopic peaks are occurred in charge 2 state both in doubly and triply charged peptide’s HCD spectrum.

keywords
CID, HCD, fragmentation pattern, multi-charged ions, LC-MS/MS


투고일Submission Date
2021-03-07
수정일Revised Date
2021-05-24
게재확정일Accepted Date
2021-05-27
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Mass Spectrometry Letters