ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

논문 상세

Home > 논문 상세
  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Metabolic Signatures of Adrenal Steroids in Preeclamptic Serum and Placenta Using Weighting Factor-Dependent Acquisitions

Metabolic Signatures of Adrenal Steroids in Preeclamptic Serum and Placenta Using Weighting Factor-Dependent Acquisitions

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2022, v.13 no.1, pp.11-19
https://doi.org/10.5478/MSL.2022.13.1.11
Chaelin Lee (Korea Institute of Science and Technology)
Min-Jeong Oh (mjohmd@korea.ac.kr)
Geum Joon Cho (Korea University Guro Hospital)
Dong Jun Byun (Korea Institute of Science and Technology)
Hong Seog Seo (Korea University Guro Hospital)
Man Ho Choi (Korea Institute of Science and Technology)
  • 다운로드 수
  • 조회수

Abstract

Although translational research is referred to clinical chemistry measures, correct weighting factors for linear and quadratic calibration curves with least-squares regression algorithm have not been carefully considered in bioanalytical assays yet. The objective of this study was to identify steroidogenic roles in preeclampsia and verify accuracy of quantitative results by comparing two different linear regression models with weighting factor of 1 and 1/x 2 . A liquid chromatography-mass spectrom-etry (LC-MS)-based adrenal steroid assay was conducted to reveal metabolic signatures of preeclampsia in both serum and pla-centa samples obtained 15 preeclamptic patients and 17 age-matched control pregnant women (33.9 ± 4.2 vs. 32.8 ± 5.6 yr, respectively) at 34~36 gestational weeks. Percent biases in the unweighted model (w i = 1) were inversely proportional to concen- trations (-739.4 ~ 852.9%) while those of weighted regression (w i = 1/x 2 ) were < 18% for all variables. The optimized LC-MS combined with the weighted linear regression resulted in significantly increased maternal serum levels of pregnenolone, 21-deoxycortisol, and tetrahydrocortisone (P < 0.05 for all) in preeclampsia. Serum metabolic ratio of (tetrahydrocortisol + allo-tet-rahydrocortisol) / tetrahydrocortisone indicating 11β-hydroxysteroid dehydrogenase type 2 was decreased (P < 0.005) in patients. In placenta, local concentrations of androstenedione were changed while its metabolic ratio to 17α-hydroxyprogester-one responsible for 17,20-lyase activity was significantly decreased in patients (P = 0.002). The current bioanalytical LC-MS assay with corrected weighting factor of 1/x 2 may provide reliable and accurate quantitative outcomes, suggesting altered ste- roidogenesis in preeclampsia patients at late gestational weeks in the third trimester.

keywords
adrenal steroid, cortisol metabolism, 17, 20-lyase, weighting factor, preeclampsia


투고일Submission Date
2022-01-07
수정일Revised Date
2022-02-11
게재확정일Accepted Date
2022-02-24
상단으로 이동

Mass Spectrometry Letters