ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

논문 상세

Home > 논문 상세
  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Effects of Column Length and Particle Diameter on Phospholipid Analysis by Nanoflow Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.3, pp.65-68
https://doi.org/10.5478/MSL.2011.2.3.065
Ju Yong Lee (Yonsei University)
Sangsoo Lim (Yonsei University)
Moon Myeong Hee (Yonsei University)
  • 다운로드 수
  • 조회수

Abstract

The effects of column length and particle size on the efficiency of separation and characterization of phospholipids(PLs) are investigated using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). Since PLs are associated with cell proliferation, apoptosis, and signal transduction, it is of increasing interests in lipidomics toestablish reliable analytical methods for the qualitative and quantitative profiling of PLs related to biomarker development inadult diseases. Due to the complexity of PLs, the preliminary separation of PLs is necessary prior to MS analysis. In this study,length of capillary column and the particle size of reversed phase (C18) packing materials are varied to find a reliable conditionfor the high speed and high resolution separation using 8 PL standard mixtures. From experiments, it was found that a capillarycolumn of nLC-ESI-MS-MS analysis for PL mixtures can be minimized to a 5 cm long pulled tip column packed with 3 μm C18particles without losing resolution.

keywords
phospholipids, nLC-ESI-MS-MS, effect of column length, particle size, capillary column


참고문헌

1

Brouwers, J. F. H. M.. (1999). . J. Lipid Res, 40, 164-.

2

Wright, M. M.. (2004). . Biochem. Cell Biol, 82, 18-.

3

Williams, C. M.. (1993). . Eur. J. Clin. Nutr, 47, 260-.

4

Sutphen, R.. (2004). . Cancer Epidemiol. Biomarkers Prev, 13, 1185-.

5

Hsu, F. F.. (2001). . J. Am. Soc. Mass Spectrom, 12, 1036-.

6

Hsu, F. F.. (2001). . J. Am. Soc. Mass Spectrom, 11, 986-.

7

Han, X.. (1995). . J. Am. Soc. Mass Spectrom, 6, 1202-.

8

Heller, D. N.. (1988). . Anal. Chem, 60, 2787-.

9

Rohlfing, A.. (2007). . Anal. Chem, 79, 5793-.

10

Kim, H. Y.. (1994). . Anal. Chem, 66, 3977-.

11

Taguchi, R.. (2005). . J. Chromatogr. B, 823, 26-.

12

Bang, D. Y.. (2006). . J. Chromatogr. A, 1104, 222-.

13

Kim, H.. (2009). . Anal. Bioanal. Chem, 393, 1649-.

14

Min, H. K.. (2010). . Anal. Bioanal. Chem, 396, 1273-.

15

Lee, J. Y.. (2011). . Anal. Bioanal. Chem, 400, 2953-.

16

Ahn, E.. (2007). . J. Separation Sci, 30, 2598-.

투고일Submission Date
2011-09-06
수정일Revised Date
2011-09-14
게재확정일Accepted Date
2011-09-14
상단으로 이동

Mass Spectrometry Letters