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  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Microwave-assisted Protein Digestion on Various Locations of a Microplate

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.4, pp.84-87
https://doi.org/10.5478/MSL.2011.2.4.084
Ji Hye Lee (Chungnam National University)
Sehwan Park (Chungnam National University)
Sunyoung Lee (Chungnam National University)
Jangmi Hong (Chungnam National University)
Kyu Hwan Park (Korea Basic Science Institute)
Kim Hyun Sik (Korea Basic Science Institute)
Jeongkwon Kim (Chungnam National University)
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Abstract

The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate wasinvestigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-wellmicroplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwaveassistedprotein digestion samples compared to conventional overnight digestion (63%), which is possibly due to higher miscleavageratios (~53%) of the samples from microwave-assisted protein digestion than conventional overnight digestion (42.1%). The reproducibleresults of microwave-assisted digestions from different well positions demonstrate the potential of high-throughput analysis ofproteins using microwave-assisted protein digestion.

keywords
Microwave, Mass spectrometry, MALDI, Microplate, Digestion


참고문헌

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투고일Submission Date
2011-12-09
수정일Revised Date
2011-12-13
게재확정일Accepted Date
2011-12-14
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Mass Spectrometry Letters