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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
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  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Optimization in Detecting Multiply-charged Protein Ions using MALDI TOF-MS

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.1, pp.21-23
https://doi.org/10.5478/MSL.2013.4.1.21
Jihyeon Lee (Chungnam National University)
Jangmi Hong (Chungnam National University)
Taehee Kim (Chungnam National University)
Jeongkwon Kim (Chungnam National University)
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Abstract

The effects of trifluoroacetic acid (TFA) were evaluated on the generation of multiply charged ions of cytochrome c in a 2-nitrophloroglucinol (2-NPG) matrix in high-vacuum, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of 1% TFA in the 2-NPG matrix solution was more effective in generating multiply charged protein ions than matrix solutions containing 0.1% or 0% TFA. Regarding the matrix itself, with 1% TFA, 2-NPG was significantly more effective in generating multiply charged ions than 2,5-dihydroxybenzoic acid (2,5-DHB). The maximum charge state of cytochrome c was +8 when using a 2-NPG matrix containing 1% TFA.

keywords
MALDI-TOF MS, 2-nitrophloroglucinol, Multiply charged ions, Laser spray ionization


참고문헌

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Miyeong Seo. (2012). Microwave-assisted Weak Acid Hydrolysis of Proteins. Mass Spectrometry Letters, 3(2), 47-49.

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투고일Submission Date
2013-03-11
수정일Revised Date
2013-03-20
게재확정일Accepted Date
2013-03-20
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Mass Spectrometry Letters