- P-ISSN 2233-4203
- E-ISSN 2093-8950
- 권한신청
- P-ISSN2233-4203
- E-ISSN2093-8950
논문 상세
Home > 논문 상세
Analysis of Phosphatidylinositol 3,4,5-Trisphosphates of PTEN Expression on Mammalian Cells
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.3, pp.41-46
https://doi.org/10.5478/MSL.2013.4.3.41
(Konkuk University)
(Korea Basic Science Institute)
(Korea Basic Science Institute)
(Konkuk University)
(Konkuk University)
(Konkuk University)
(Konkuk University)
(Korea Basic Science Institute)
(Korea Basic Science Institute)
(Konkuk University)
(Konkuk University)
(Konkuk University)
(Konkuk University)
Jahan,
N.,
Park,
T.,
Kim,
Y. H.,
Lee,
D.,
Kim,
H.,
Noh,
K.,
&
Kim,
Y.
J.
(2013). . Mass Spectrometry Letters, 4(3), 41-46, https://doi.org/10.5478/MSL.2013.4.3.41
- 다운로드 수
- 조회수
Abstract
The goal of this study is to find an experimental condition which enables us to perform enzymatic studies on the cellularbehavior of PTEN (phosphatase and tensine homolog) through identification of molecular species of phosphatidylinositol 3,4,5-trisphosphates and their quantitative analysis in a mammalian cell line using mass spectrometry. We initially exployed a two-stepextraction process using HCl for extraction of phosphatidylinositol 3,4,5-trisphosphates from two mammalian cell lines and furtheranalyzed the extracted phosphatidylinositol 3,4,5-trisphosphates using tandem mass spectrometry for the identification of them. Wefinally quantified the concentration of phosphatidylinositol 3,4,5-trisphosphates using internal standard calibration. From theseobservation, we found that HEK 293-T cells is a good model to examine the enzymatic behavior of PTEN in a cell, and theminimum amount of phosphatidylinositol 3,4,5-trisphosphates is more than 50 pmol for quantification in a mass spectrometer. These results suggest that the well-optimized experimental conditions are required for the investigation of the cellular PTEN interms of the catalytic mechanism and further for the detailed identification of cellular substrates.
- keywords
- Phosphoinositides, Lipidomics, PTEN, Mass Spectrometry, Enzymatics
참고문헌
1
Vanhaesebroeck, B. (2001). . Annu. Rev. Biochem, 70, 535-.
2
Arendt, K. L. (2010). . Nat. Neurosci, 13, 36-.
3
Wenk, M. R. (2003). . Nat. Biotechnol, 21, 813-.
4
Stephens, L. R. (1993). . Biochim. Biophys. Acta, 1179, 27-.
5
Maehama, T. (1998). . J. Biol. Chem, 273, 13375-.
6
Milne, S. B. (2005). . J. Lipid Res, 46, 1796-.
7
Hawkins, P. T. (1992). . Nature, 358, 157-.
8
Rameh, L. E. (1999). . J. Biol. Chem, 274, 8347-.
9
Lemmon, M. A.. (2003). . Traffic, 4, 201-.
10
Kashiwada, M. (2007). . Immunol. Res, 39, 194-.
11
Chang, H. W. (1997). . Science, 276, 1848-.
12
Jimenez, C. (1998). . EMBO J, 17, 743-.
13
Myers, M. P. (1998). . Proc Natl Acad Sci. U S A, 95, 13513-.
14
Wang, X. (2000). . J. Immunol, 164, 1934-.
15
Li, J. (1997). . Parsons, R. Science, 275, 1943-.
16
Hlobilkova, A. (2003). . Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub, 147, 19-.
17
Stambolic, V. (1998). . Cell, 95, 29-.
18
Lee, J. O. (1999). . Cell, 99, 323-.
19
Li, D. M. (1998). . Proc. Natl Acad Sci. USA, 95, 15406-.
20
Leslie, N. R. (2003). . EMBO J, 22, 5501-.
21
Gray, A. (1999). . Biochem. J, 3(344 Pt), 929-.
22
Alter, C. A. (1995). . Biochem. Biophys. Res. Commun, 208, 190-.
23
Nasuhoglu, C. (2002). . Anal. Biochem, 301, 243-.
24
김영준. (2010). Mass spectrometry based cellular phosphoinositides profiling and phospholipid analysis: A brief review. Experimental and Molecular Medicine, 42(1), 1-11.
25
Conway S. J. (2010). . Org. Biomol. Chem, 8, 66-.
- 투고일Submission Date
- 2013-07-22
- 수정일Revised Date
- 2013-09-24
- 게재확정일Accepted Date
- 2013-09-24
권호 내 다른 논문
- Analysis of Phosphatidylinositol 3,4,5-Trisphosphates of PTEN Expression on Mammalian Cells
- Ultra-fast Generic LC-MS/MS Method for High-Throughput Quantification in Drug Discovery
- Isotopic Analysis of NUSIMEP-6 Uranium Particles using SEM-TIMS
- Liquid Chromatography-tandem Mass Spectrometry for Quantification of Dioscin in Rat Plasma
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.