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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Stabilization of Compact Protein Structures by Macrocyclic Hosts Cucurbit[n]urils in the Gas Phase

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.1, pp.16-20
https://doi.org/10.5478/MSL.2016.7.1.16
Jong Wha Lee (Pohang University of Science and Technology (POSTEC), Korea University)
Mi Hyun Park (Kyeongbuk Science High School)
Jeong Tae Ju (Kyeongbuk Science High School)
Yun Seop Choi (Kyeongbuk Science High School)
Soo Min Hwang (Kyeongbuk Science High School)
Dong Jin Jung (Kyeongbuk Science High School)
Hugh I. Kim (Korea University)
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Abstract

Characterization of intact protein structures in the gas phase using electrospray ionization combined with ion mobility mass spectrometry has become an important tool of research. However, the biophysical properties that govern the structures of protein ions in the gas phase remain to be understood. Here, we investigated the impact of host-guest complexation of ubiquitin (Ubq) with macrocyclic host molecules, cucurbit[n]urils (CB[n]s, n = 6, 7), on its structure in the gas phase. We found that CB[n] complexation induces the formation of compact Ubq ions. Both CB[6] and CB[7] exhibited similar effects despite differences in their binding properties in solution. In addition, CB[n] attachment prevented Ubq from unfolding by collisional activation. Based on the experimental results, we suggest that CB[n]s prevent unfolding of Ubq during transfer to the gas phase to promote the formation of compact protein ions. Furthermore, interaction with positively charged residues per se is suggested to be the most important factor for the host-guest complexation effect.

keywords
ion mobility mass spectrometry, cucurbit[n]uril, ubiquitin, host-guest chemistry


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투고일Submission Date
2015-12-19
수정일Revised Date
2016-01-23
게재확정일Accepted Date
2016-01-26
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Mass Spectrometry Letters