Effect of Cationization Agent Concentration on Glycan Detection Using MALDI TOF-MS

Kim Inyoung; Shin Dongwon; Paek Jihyun; Kim Jeongkwon

doi:10.5478/MSL.2017.8.1.14

P-ISSN2233-4203
E-ISSN2093-8950
ESCI, SCOPUS, KCI

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P-ISSN 2233-4203
E-ISSN 2093-8950

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Vol.8, No.1

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Effect of Cationization Agent Concentration on Glycan Detection Using MALDI TOF-MS

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950

2017, v.8 no.1, pp.14-17

https://doi.org/10.5478/MSL.2017.8.1.14

Kim Inyoung (Chungnam National University)
Shin Dongwon (Chungnam National University)
Paek Jihyun (Chungnam National University)
Kim Jeongkwon (Chungnam National University)

Kim, I., Shin, D., Paek, J., & Kim, J. (2017). Effect of Cationization Agent Concentration on Glycan Detection Using MALDI TOF-MS. Mass Spectrometry Letters, 8(1), 14-17, https://doi.org/10.5478/MSL.2017.8.1.14

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Abstract

The effect of cationization agent concentration on glycan detection via matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF MS) was investigated using Na+ ions in the form of NaCl as the cationization agent. NaCl solution concentrations ranging from 1 mM to 1 M were investigated. Glycans from ovalbumin were mixed with the cationization agent solution and the 2,5-dihydroxybenzoic acid (2,5-DHB) matrix solution in a volume ratio of 1:1:1. The resulting mixture was loaded onto the MALDI plate. Two MALDI-TOF MS instruments (Voyager DE-STR MALDI-TOF MS and Tinkerbell RT MALDITOF MS) were used for detection of glycans. The best detection, in terms of the number of identified glycans, the peak intensity, and the signal-to-noise (S/N) ratio, was obtained with NaCl concentrations of 0.01–0.1 M for both MALDI-TOF MS instruments.

keywords: MALDI-TOF MS, glycans, cationization agent, PNGase F, 2, 5-dihydroxybenzoic acid

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