![](/msletters/assets/coverImage/J000011_cover.jpg)
- P-ISSN 2233-4203
- E-ISSN 2093-8950
Glycated hemoglobin (HbA 1c ) has been commonly used to screen and diagnose for patients with diabetes mellitus. Here the accelerated procedure of microwave-assisted sample treatment from acid hydrolysis to enzyme digestion followed by isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) was optimized and applied to measure HbA 1c in an effort to speed up analysis time. First, two signature peptides of HbA 1c and hemoglobin A 0 were certified with amino acid analysis by setting optimized acid hydrolysis conditions to 150 o C, 1.5 h and 10 µM sample concentration in 8 M hydrochloric acid. Consequently, the accurate certified peptides above were used as calibration standards to implement the pro-teolytic procedure with endoproteinase Glu-C at 37 o C, 700 W for 6 h. Compared to the traditional method, the microwave heat-ing not only shortened dramatically sample preparation time, but also afforded comparable recovery yields. The optimized protocol and analytical conditions in this study are suitable for a primary reference method of HbA 1c quantification with full SI-traceability and other similar proteins in complex biological samples.
Peterson, K. P. (1998). . Clin. Chem, 44, 1951-.
Higgins, T. (2012). . Clin. Biochem, 45, 1038-.
. (2011). World Health Organization Use of Glycated Haemoglobin (HbA1c) in the Diagnosis of Diabetes Mellitus - Abbreviated Report of a WHO Consultation.
Jeppsson, J. O. (2002). International Federation of Clinical Chemistry and Laboratory Medicine Clin. Chem. Lab. Med, 40, 78-.
Kaiser, P. (2010). . Clin. Chem, 56, 750-.
Chen, S. T. (1987). . Int. J. Pept. Protein Res, 30, 572-.
Weiss, M. (1998). . J. Chromatogr. A, 795, 263-.
Engelhart, W. G. (1990). . Am. Biotechnol. Lab, 8, 30-.
Mishra, A. (2015). Microwave-Assisted Polymerization:Royal Society of Chemistry.
Rejasse, B. (2007). . J. Enzyme Inhib. Med. Chem, 22, 518-.
Tran, T. T. H. (2017). . J. Chromatogr. A, 1513, 183-.
Pritchard, C. (2011). . Anal. Biochem, 412, 40-.
Fountoulakis, M. (1998). . J. Chromatogr. A, 826, 109-.
Damm, M. (2010). . J. Chromatogr. A, 1217, 7826-.