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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

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  • P-ISSN2799-2977
  • E-ISSN2799-4066

부자 추출물이 LPS로 유도된 C57BL6 마우스의 패혈증 연관 급성 폐 손상에 미치는 영향

Effect of Water Extract of Aconiti Lateralis Preparata Radix on Lung Injury in LPS-induced Septic C57BL6 Mice

대한융합한의학회지 / Journal of convergence Korean Medicine, (P)2799-2977; (E)2799-4066
2022, v.4 no.1, pp.19-27
이인승 (동국대학교 한의과대학 병리학교실)
부민아 (경희대학교 한의과대학 약리학교실)
심재욱 (경희대학교 한의과대학 약리학교실)
백승호 (동국대학교 한의과대학 병리학교실)
박진봉 (경희대학교 한의과대학 약리학교실)

Abstract

Objectives: TSepsis and subsequent acute lung injury (ALI) is a critical state of health caused by infection or endotoxins. This study was conducted to evaluate the effect of Water Extract of Aconiti Lateralis Preparata Radix (AR) on lipopolysaccharide (LPS)-induced sepsis in C57BL/6 mice. Methods: Male C57BL/6 mice were intraperitoneally injected with LPS to induce sepsis and ALI. AR was orally fed twice at 30 min and 180 min after LPS injection. At 24 h post injection, mice were sacrificed, bronchoalveolar lavage fluid (BALF) and blood was collected, and lung tissue was harvested. Hematoxylin and eosin staining was performed in lung tissues, wet/dry ratio of the lung tissue was measured, and the serum cytokine and chemokine levels were analyzed. Results: AR revoked the LPS-induced pathological changes in lung tissues, such as abnormal histological structures, immune cell infiltration and lung edema. Also, AR suppressed the neutrophil infiltration into the lung which was greatly increased by LPS injection based on the cell content of collected BALF. Serum cytokines and chemokines were measured, and AR reversed the LPS-induced increase of cytokines such as interleukin 1 beta, interleukin 6, tumor necrosis factor alpha and chemokines including C-X-C motif chemokine ligand 1 and 2. Conclusion: TAR showed a protective effect in the pathological progress of LPS-induced ALI. Especially, AR suppressed lung edema and infiltration of neutrophils by inhibiting cytokine and chemokine expressions. Such results demonstrate the potential of AR as a therapeutic agent for sepsis and sepsis-induced ALI.

keywords
Sepsis, Acute Lung Injury, Lipopolysaccharide, Aconitii Laterails Perparata Radix

대한융합한의학회지