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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

고지방식이를 급여한 마우스의 간과 HepG2 세포에서 TJGB의 효과에 대한 연구

Effect of TJGB on the liver of high-fat diet-fed mice and the viability of HepG2 cells

대한융합한의학회지 / Journal of convergence Korean Medicine,
2023, v.5 no.1, pp.55-60
김희영 (경희대학교 약학대학 천연물융합신약개발학과)
박예진 (상지대학교 한의과대학 한방재활의학과)
안효진 (경희대학교 약학대학 천연물융합신약개발학과)

Abstract

Objectives: This study was performed to investigate the effect of TJGB on the liver of high-fat diet (HFD)-fed mice and the cell viability of HepG2 cells. Methods: After a week adaptation, 8-week-old C57BL/6N mice were fed with a 45% HFD or normal diet for 3 weeks. For the next 9 weeks, the mice were divided into 6 groups: normal diet group; HFD group; HFD plus orlistat group; HFD plus Ephedra sinica Stapf (ES) group; HFD plus low dose of TJGB group; HFD plus high dose of TJGB group. To estimate the effect of TJGB in the liver of HFD-fed mice, the protein expressions of phospho-acetyl-CoA carboxylase (p-ACC) and liver X Receptor (LXR) were determined by Western blot assay. The cell viability of ES and TJG was also evaluated in HepG2 cells. Results: The administration of TJGB had little effect on the protein expressions of p-ACC and LXR in the liver of HFD-fed mice. And the cytotoxicity was showed above 7.8 ㎍/mL in HepG2 cells. Conclusion: Further research is needed to evaluate the mechanism of TJGB on hepatic steatosis and cytotoxicity in HepG2 cells.

keywords
High-fat diet, HepG2 cells, Obesity, Liver, TJGB

대한융합한의학회지