- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
ROS-, RNS-Scavenging and Anti-inflammatory Activities of Mori Fructus
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.1, pp.106-116
&
(2008). ROS-, RNS-Scavenging and Anti-inflammatory Activities of Mori Fructus. Journal of Korean Medicine, 29(1), 106-116.
- Downloaded
- Viewed
Abstract
Objectives:Peroxynitrite (ONOO-), superoxide anion radical (․O2-) and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate the ONOO-, NO, and ․O2- scavenging and anti-inflammatory activities of Mori Fructus in ob/ob mice. Methods:Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups received the standard chow. The experimental groups were fed a diet of chow supplemented with 7.5, 15 and 30 mg Mori Fructus per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho IκB-α, anti-IKK-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS respectively. Results:Mori Fructus inhibited the generation of ONOO-, NO and ․O2- in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondria in vitro. The generation of ONOO-, NO and ․O2- were inhibited in the Mori Fructus-administered ob/ob mice groups. The GSH/GSSG ratio decreased in the ob/ob mice, whereas they improved in the Mori Fructus-administered groups. Mori Fructus inhibited the expression of phospho IκB-α, IKK-α, COX-2, iNOS genes, and thereby the activation of NF-κB. Conclusions:These results suggest that Mori Fructus is an effective ONOO-, ․O2- and NO scavenger, and therefore it might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.
- keywords
- Mori Fructus, peroxynitrite, nitric oxide, reactive oxygen species, NF-κB, COX-2, iNOS
Reference
1
1. De Martinis M, Franceschi C, Monti D, Ginaldi L. Inflamma-ageing and lifelong antig- enic load as major determinants of ageing rate and longevity. FEBS Lett. 2005;579(10):2035-9.
2
2. Ginaldi L, De Martinis M, Monti D, Franc- eschi C. Chronic antlgenic load and apoptosis in immunosenescence. trends Immunol. 2005; 26:79-84.
3
3. Virag LE, Szabo P, Gergely and C szabo. Peroxynitrite induced cytotoxicity: mechanism and opportunities for intervention. Toxicol Lett. 2003;140-1:113-24.
4
4. Balavoine GGA and YV Geletii. Peroxynitrite scavenging by different antioxidants part 1: convenient assay, Nitric Oxide: Biology and Chemistry. 1999;3:40-54.
5
5. Chung HY, Kim HJ, Jung KJ, Yoon JS, Yoo MA, Kim KW, Yu BP. The inflammatory process in aging. Reviews in Clinical Geron- tology. 2000;10:207-22.
6
6. Chung HY, Kim HJ, and Kim JW. The inflammation hypothesis of aging : Molecular modulation by calorie restriction. Ann. N. Y. Acad. Sci. 2001;928:327-35.
7
7. Kim HJ, Kim KW, Yu BP, Chung HY. The effect of age on cyclooxygenase-2 gene expre- ssion : NF-κB activation and IκBα degra- dation. Free Radical Biol Med. 2000;28:683 -92.
8
8. Ghosh S, May MJ, Kopp EB. NF-κB and Rel proteins : Evolutionarity conserved mediators of immune responses. Annu Rev Immunol. 1998;16:225-60.
9
9. Luo SF, Wang CC, Chien CS, Hsiao LD, Yang CM, Induction of cyclooxygenase-2 by lipopo- lysaccharide in canine tracheal smooth muscle cells : invilvement of p42/p44 and p38 mitogen- activated protein kinases and nuclear factor- kappa B pathways. 2003;15:497-509.
10
10. Korhonen P, Helenius M, Salminen A. Age- related changes in the regulation of transcr- iption factor NF-kappa B in rat brain. Neurosci Lett. 1997;225:61-4.
11
11. 江蘇新醫學院 編. 中藥大辭典. 上海:上海科學技術出版社;1983, p.1967-8.
12
12. 全國韓醫科大學 本草學敎授 共編. 本草學. 서울:永林社;1991, p.598-9.
13
13. 곽영, 신길조, 조기호, 김영석, 배형섭, 이경섭. 桑枝, 桑葉, 桑白皮 및 桑椹子의 항지혈 효과에 관한 연구. 경희의학. 1992;8(1):44.
14
14. 박용기, 강병수. 桑椹子의 항산화작용에 관한 연구. 대한본초학회지. 1999;14(2):43-50.
15
15. 황금희. 상심자(Morus alba)의 운동능력 향상과 스트레스 개선 효과. 한국식품과학회지. 2005;37(1):95-102.
16
16. 許 浚. 東醫寶鑑. 서울:南山堂;1981, p.80.
17
17. 이동준, 정지천. 三精丸의 Peroxynitrite 제거 효과. 대한한방내과학회지. 2006;27(1):178- 87.
18
18. 정해주, 김세윤, 정지천. 고지방 식이 흰쥐의 비만에 미치는 三精丸의 영향. 대한한의학회지. 2006;27(3):516-27.
19
19. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Res Commun. 1994;16:149-56.
20
20. Nagata N, Momose K, Ishida Y. Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reaction of 4,5-diamino- fluorescein, DAF-2, a novel indicator of nitric oxide. J Biochem Tokyo. 1999;125:658-61.
21
21. Cathcart R, Schwiers E, Ames BN. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay. Anal Biochem. 1983;134:111-6.
22
22. Gaitonide MK. A spectrophotometric method for the direct determination of cystein in the presense of other naturally occuring amino acid. Biochem. J. 1967;104:627.
23
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951;193:265-75.
24
24. 金完熙, 崔達永 編著. 臟腑辨證論治. 서울:成輔社;1985, p.282-4.
25
25. 王其飛 外. 中醫長壽學. 遼寧:遼寧科學技術出版社;1989, p.53-4, 327-9, 331-4.
26
26. Collins T. Endothelial nuclear factor-kappa B and the initiation of the atherosclerotic lesion. Lab Invest. 1993;68:499-503.
27
27. Chung HY, Kim HJ, Shim KH, Kim KW. Dietary modulation of prostanoid synthesis in the aging process : role of cyclooxygenase-2. Mech Ageing Dev. 1999;111:97-106.
28
28. Mordes JP and Rossini AA. Animal models of diabetes mellitus. Am J Med. 1981;70(2) :353-60.
29
29. Eleazar S. Animal models of non-insulin-depeent diabetes. Diabetes metabolism reviews. 1992;8 (3):179-208.
30
30. Cai H, Harrison DG. Endothelial dysfunction in cardiovascular diseases ; the role of oxidant stress. Circ Res. 2000;87:840-4.
31
31. Matsubara T, Ziff M. Increased syperoxide anion release from human endothelial cells in response to cytokines. J Immunol. 1986;137: 3295-8.
32
32. Vendemiale G, Altomare E, Grattagliano I, Albano O. Increased plasma levels of glutat- hione and malondialdehyde after avute ethanol ingestion in humans. J hepatol. 1989;9:359.
33
33. Chung HY, Soung DY, Kim AR, Choi HR, Kim HJ, Choi JS, Yang R, Lee KH and Yu BP. Generation, Toxicity and Scavenging of ONOO-: Its Involvement in the Aging Process. Kor J Gerontol. 2000;10:46-59.
34
34. Groszmann RJ. Hyperdynamic state in chronic liver disease. J Hepatol. 1993;17(2): S38-40.
35
35. Tsujii M, Kawano S, Tsuji S, Sawaoka H, Hori M, DuBois RN. Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell. 1998;93(5):705-16.
36
36. Chung HY, Kim HJ, Kim KW, Chio JS, Yu BP. Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction. Micro Res Techinq. 2002 ;59:264-72.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- A Crossover Clinical Trial to Determine the Effect of Siguan(Four Gates) Points on Gastrointestinal Motility Suppressed by Loperamide Administration
- Multi-facet Analysis on Validity of Sasang Type Diagnostic Test
- A Study on the Problem about Oriental Treatment Fee Based on Valuation of Resources
- Development of Complementary and Alternative Medicine Curriculum for Undergraduate Students at College of Oriental Medicine
- Acupuncture on ST36 Increases c-Fos Expression in vlPAG of Visceral Pain-induced Mice
- The Inhibitory Effect of Lycii Fructus on LPS-stimulated NF-κB Activation and iNOS Expression in RAW 264.7 Macrophages
- Systematic Analysis of Ginseng-focused Research Worldwide
- Effect of Jasinwhalhyul-tang on MRL/MpJ-Ipr/Ipr Mouse Model with Systemic Lupus Erythematosus
- Study of Effects and Optimum Concentrations of Medical Herb Extracts on Blood Glucose and Serum Lipid in Streptozotocin-Induced Diabetic Rats
- Effect of Polygonati Sibirici Rhizoma on Cell Viability in Human Glioma Cells
- ROS-, RNS-Scavenging and Anti-inflammatory Activities of Mori Fructus
- Skin Humidity Effects of Strontium on Acute Skin Barrier Damage in Hairless Mice
- A Study of the Relationship between Drinking, Smoking and Stress of Workers by Analyzing Heart Rate Variability
- 14The effectiveness of oriental medical therapy compared to oriental-western medical therapy on acute bell’s palsy
- Study on Pharmacological Activity of Sipjeondaebotang by Difference in Component Ratio between Astragali Radix and Cinnamomi Cortex
- The Effect of the Water Extract of Angelica Sinens on Gliosis Repression of Astrocyte after Hypoxic injury
- Therapeutic Effects of Cheonggi-san Extract on NC/Nga Mice with Atopic Dermatitis-like Skin Lesions
- Study of Silent Infarct in First-ever Cerebral Infarction Patients Treated in an Oriental Medical Hospital.
- A Study on Deficiency Syndrome, Excess Syndrome, Reinforcing Method and Reducing Method
- 12 Cases of Strabismus Treated by Acupuncture and Herb Medicine
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.