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  • E-ISSN2288-3339

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桑子추출물의 ROS, RNS 및 염증 촉진 인자 제어 효과

ROS-, RNS-Scavenging and Anti-inflammatory Activities of Mori Fructus

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.1, pp.106-116
박순재 (동국대학교)
정지천 (동국대학교)
박순재, & 정지천. (2008). 桑子추출물의 ROS, RNS 및 염증 촉진 인자 제어 효과. , 29(1), 106-116.
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Abstract

Objectives:Peroxynitrite (ONOO-), superoxide anion radical (․O2-) and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate the ONOO-, NO, and ․O2- scavenging and anti-inflammatory activities of Mori Fructus in ob/ob mice. Methods:Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups received the standard chow. The experimental groups were fed a diet of chow supplemented with 7.5, 15 and 30 mg Mori Fructus per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho IκB-α, anti-IKK-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS respectively. Results:Mori Fructus inhibited the generation of ONOO-, NO and ․O2- in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondria in vitro. The generation of ONOO-, NO and ․O2- were inhibited in the Mori Fructus-administered ob/ob mice groups. The GSH/GSSG ratio decreased in the ob/ob mice, whereas they improved in the Mori Fructus-administered groups. Mori Fructus inhibited the expression of phospho IκB-α, IKK-α, COX-2, iNOS genes, and thereby the activation of NF-κB. Conclusions:These results suggest that Mori Fructus is an effective ONOO-, ․O2- and NO scavenger, and therefore it might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.

keywords
Mori Fructus, peroxynitrite, nitric oxide, reactive oxygen species, NF-κB, COX-2, iNOS


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