Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Bacterial Reverse Mutation Test of Sipjeondaebo-tang

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2008, v.29 no.3, pp.1-10






  • Downloaded
  • Viewed

Abstract

Objectives:This study was to assess the toxicity of Sipjeondaebo-tang by bacterial reverse mutation test. Methods:In this study, to evaluate the bacterial reverse mutation of Sipjeondaebo-tang water-extract, the in vitro Ames test using Salmonella typhimurium (TA98, TA100, TA1,535, TA1,537) and Escherichia coli(WP2uvrA) were performed with Sipjeondaebo-tang water extract at the concentrations 0, 312, 625, 1250, 2500 and 5000 ㎍/plate. Results:Sipjeondaebo-tang water extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S9- fraction and S+ fraction). Conclusions:According to these results, we concluded that a Sipjeondaebo-tang water extract did not cause bacterial reverse mutation.

keywords
Sipjeondaebo-tang, reverse mutation, Ames test, Escherichia coli. Shiquan dabu-decoction


Reference

1

1. Saiki I. A Kampo medicine ‘'Juzen-taiho-to’' -prevention of malignant progression and metastasis of tumor cells and the mechanism of action. Biol Pharm Bull 2000;23:677-688.

2

2. Ohnishi Y. Fujii H. Hayakawa Y. Yamamura T. Sakamoto T. Tsukada K. Fujimaki M. Nunome S. Komatsu Y. Saiki I. Oral adminis- tration of a Kampo (Japanese herbal) medicine Juzen-taiho-to inhibits liver metastasis of colon 26-L5 carcinoma cells. Jpn. J. Cancer Res:1998;89:206-213.

3

3. Saiki I. Yamamura T. Ohnishi Y. Hayakawa Y. Komatsu Y. Nunome S. HPLC analysis of juzen-taiho-to and its variant formulations and their antimetastatic efficacies. Chem. Pharm. Bull:1999;47:1170-1174.

4

4. Matsumoto T. Sakurai M. H. Kiyohara H. Yamada H. Immunopharmacol. Orally admini- stered decoction of Kampo (Japanese herbal) medicine, “Juzen-Taiho-To” modulates cytokine secretion and induces NKT cells in mouse liver. Immunopharmacol:2000;46,149-161.

5

5. Miyagami M. Katayama Y. Improvement of host-immunity by adjuvant therapy with juzen- taiho-to for patients with brain tumors. No Shinkei Geka:2003;31:401-409.

6

6. Utsuyama M. Seidlar H. Kitagawa M. Hiro- kawa K. Immunological restoration and anti- tumor effect by Japanese herbal medicine in aged mice. Mech. Ageing Dev:2001;122:341 -352.

7

7. Ohnishi Y, Fujil H, Kimura F, et al. Inhibitory effect of traditional Chinese medicine Juzent- ailio-to on progressive growth of weakly malignant clone cells derived from murine fibrosarcoma. Jpn J Cancer Res:1996;87:1039 -1044.

8

8. Maruyama H, Takemoto N, Maruyama N, Kornatsu Y, Kawamura H. Antitumor effect of Juzen-taiho-to, a kampo medicine, combined with surgical excision of transplanted Meth-A fibrosarcoma. Int J Immunother:1993;9:117 -125.

9

9. Zhang YH, Kato M, Isobe K, Hamaguchi M, Yokochi T, Nakashima I. Dissociated control by glycyrrhizin of proliferation and IL-2 production of murine thymocytes. Cell Immu- nol.1995:162;97-104.

10

10. Ohnishi Y, Fujil H, Kimura F, et al: Inhibitory effect of traditional Chinese medicine Juzentailio -to on progressive growth of weakly malignant clone cells derived from murine fibrosarcoma. Jpn J Cancer Res.1996:87;1039-1044.(423)

11

11. Hisha H, Yamada H, Sakurai MH, Kiyohara H, Li Y, Yu C, Takemoto N, Kawamura H, Yamaura K, Shinohara S,Komatsu Y, Aburada M, Ikehara S. Isolation and identification of hematopoietic stem cell-stimulating substances from Kampo (Japanese herbal) medicine, Juzen-taiho-to. Blood:1997;90:1022-1030.

12

12. Dai Y, Kato M, Takeda K, Kawamoto Y, Akhand AA, Hossain K, Suzuki H, Nakashima I. T-cell-immunity-based inhibitory effects of orally administered herbal medicine juzen- taiho-to on the growth of primarily developed melanocytic tumors in RET-transgenic mice. J Invest Dermatol:2001;117:694-701.

13

13. Kiyohara H, Yamada H, Takemoto N, Kaw- amura H, Komatsu Y, Oyama T. Character- ization of in vitro IL-2-production-enhancing and anticomplementary pectic polysaccharides from Kampo (Japanese herbal) medicine Juzen- taiho-to. Phytotherapy Res:1993;7:367-375.

14

14. Iijima K, Sun S, Cyong JC, Jyonouchi H: Juzen-taiho-to, a Japanese herbal medicine, modulates type-1 and type-2 T cell responses in old BALB/c mice. Am J Chin Med:1999; 27:191-203.

15

15. Matsumoto T, Yamada H: Orally administered kampo (Japanese herbal) medicine Juzen-taiho -to modulates cytokine secretion in gut associated lymphoreticular tissues in mice. Phytomedicine:2000;6:425-430.

16

16. Matsumoto T, Matsumi H, Sakurai H, Kiyohara H, Yamada H. Orally dministered decoction of kampo (Japanese herbal) medicine Juzen-taiho-to modulates cytokine secretion and induces NKT cells in mouse liver. Immun- ophamacology:2000;46:149-161.

17

17. 송시환, 양덕춘, 정세영. 산삼배양추출물의 세균을 이용한 복귀돌연변이 시험. J.Fd Hyg. Safety 2004;19(4),193-107(424)

18

18. Maron, D.M. and Ames, B.N., Revised Meth- ods for the Salmonella Mutagenicity Test. Mutation Res. 1983;113, 173-215

19

19. 비임상시험관리기준(식품의약품안전청고시 제 2005-60호, 2005)

20

20. OECD guidelines for the testing of chemicals, No. 471 ‘Bacterial Reverse Mutation Test’ (1997)

  • Downloaded
  • Viewed
  • 0KCI Citations
  • 0WOS Citations

Other articles from this issue

Recommanded Articles

상단으로 이동

Journal of Korean Medicine